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Article has an altmetric score of 6

Grant support

This work has been supported under the Project INNBIOD (REF: IPT-2011-1429-010000) under the Spanish Ministry of Economy and Competitiveness (INNPACT program), the Project PLATON (REF: TEC2012-31145) under the Spanish Ministry of Economy and competitiveness (Plan Nacional 2012 Investigacion fundamental no orientada) and ENVIGUARD EUROPEAN PROJECT FP7-OCEAN-2013. We acknowledge specially Bioftalmik and Antibody Bcn for theirtechnical and scientific support, as well as for the bio-chemical reagents to carry out the experiments described in this article.

Analysis of institutional authors

Laguna MCorresponding AuthorHolgado MAuthorHernández AlAuthorSantamaria BAuthorLavín áAuthorSanza FjAuthorCasquel RAuthor

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Article

Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing

Publicated to:Sensors. 15 (8): 19819-29 - 2015-08-13 15(8), DOI: 10.3390/s150819819

Authors: Laguna, Mariafe; Holgado, Miguel; Hernandez, Ana L; Santamaria, Beatriz; Lavin, Alvaro; Soria, Javier; Suarez, Tatiana; Bardina, Carlota; Jara, Monica; Sanza, Francisco J; Casquel, Rafael

Affiliations

AntibodyBcn, Barcelona 08193, Spain - Author
Bioftalmik, Bizkaia 48160, Spain - Author
BioOpticalDetection, Centro de Empresas de la UPM, Campus Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain - Author
Campus Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain - Author
Center for Biomedical Technology, Optics, Photonics and Biophotonics Lab, Universidad Politécnica de Madrid - Author
Department of Applied Physics and Material, Escuela Técnica Superior de Ingenieros Industriales (ETSII), Universidad Politécnica de Madrid, Jose Gutiérrez Abascal, 2 - Author
Grupo de Investigación en Ingeniería y Aplicaciones del Láser. Universidad Politécnica de Madrid - Author
M - Author
Univ Politecn Madrid, Ctr Biomed Technol, Opt Photon & Biophoton Lab, Madrid 28223, Spain - Author
Univ Politecn Madrid, Dept Appl Phys & Mat, ETSII, E-28006 Madrid, Spain - Author
Upm - Author
UPM, Ctr Empresas, BioOpticalDetect, Madrid 28223, Spain - Author
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Abstract

The specificity and affinity of antibody-antigen interactions is a fundamental way to achieve reliable biosensing responses. Different proteins involved with dry eye dysfunction: ANXA1, ANXA11, CST4, PRDX5, PLAA and S100A6; were validated as biomarkers. In this work several antibodies were tested for ANXA1, ANXA11 and PRDX5 to select the best candidates for each biomarker. The results were obtained by using Biophotonic Sensing Cells (BICELLs) as an efficient methodology for label-free biosensing and compared with the Enzyme-Linked Immuno Sorbent Assay (ELISA) technique.

Keywords

AnimalsAnnexinsAntibodies, monoclonalAntibody affinityAntigensBiomarkersBiosensing techniquesBiosensorsCalibrationDry eye biomarkersDry eye syndromesEnzyme-linked immunosorbent assayFemaleKineticsLabel-free immunoassayMice, inbred balb cOptical phenomenaPhotonic sensing cells bicellsStaining and labelingSu-8

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Sensors due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2015, it was in position 12/56, thus managing to position itself as a Q1 (Primer Cuartil), in the category Instruments & Instrumentation.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 2.45, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions Jun 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-06-10, the following number of citations:

  • WoS: 11
  • Scopus: 11
  • Europe PMC: 7
  • OpenCitations: 10

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-06-10:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 40.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 40 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 6.

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (LAGUNA HERAS, MARIA FE) and Last Author (CASQUEL DEL CAMPO, RAFAEL).

the author responsible for correspondence tasks has been LAGUNA HERAS, MARIA FE.