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Analysis of institutional authors

Martín-Dacal MAuthorFernández-Calvo PCorresponding AuthorLopez GAuthorGarrido-Arandia MAuthorRebaque DAuthorDel Hierro IAuthorBerlanga DjAuthorTorres MaAuthorKumar, VarunAuthorMélida HAuthorPacios LfAuthorMolina, ACorresponding Author

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February 13, 2023
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Arabidopsis immune responses triggered by cellulose- and mixed-linked glucan-derived oligosaccharides require a group of leucine-rich repeat malectin receptor kinases

Publicated to: PLANT JOURNAL. 113 (4): 833-850 - 2023-02-01 113(4), DOI: 10.1111/tpj.16088

Authors:

Martin-Dacal, Marina; Fernandez-Calvo, Patricia; Jimenez-Sandoval, Pedro; Lopez, Gemma; Garrido-Arandia, Maria; Rebaque, Diego; del Hierro, Irene; Berlanga, Diego Jose; Torres, Miguel Angel; Kumar, Varun; Melida, Hugo; Pacios, Luis F F; Santiago, Julia; Molina, Antonio
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Affiliations

CSIC-UPM-INIA-CBGP - Centro de Biotecnología y Genómica de Plantas - Author
CSIC-UPM-INIA-CBGP - Centro de Biotecnología y Genómica de Plantas , Alimentaria y de Biosistemas - Author
Royal Inst Technol KTH, Sch Biotechnol, Div Glycosci, Stockholm, Sweden - Author
Univ Lausanne UNIL, Dept Biol Mol Vegetale DBMV, UNIL Sorge, Biophore Bldg, CH-1015 Lausanne, Switzerland - Author
Univ Leon, Dept Ingn & Ciencias Agr, Area Fisiol Vegetal, Leon, Spain - Author
Univ Politecn Madrid UPM, Ctr Biotecnol & Genom Plantas, Inst Nacl Invest & Tecnol Agr & Alimentaria INIA, Campus Montegancedo UPM, Pozuelo De Alarcon 28223, Spain - Author
Université de Lausanne (UNIL) - Author
UPM, Escuela Tecn Super Ingn Agron Alimentaria & Biosi, Dept Biotecnol Biol Vegetal, Madrid 28040, Spain - Author
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Abstract

The plant immune system perceives a diversity of carbohydrate ligands from plant and microbial cell walls through the extracellular ectodomains (ECDs) of pattern recognition receptors (PRRs), which activate pattern-triggered immunity (PTI). Among these ligands are oligosaccharides derived from mixed-linked β-1,3/β-1,4-glucans (MLGs; e.g. β-1,4-D-(Glc)2-β-1,3-D-Glc, MLG43) and cellulose (e.g. β-1,4-D-(Glc)3, CEL3). The mechanisms behind carbohydrate perception in plants are poorly characterized except for fungal chitin oligosaccharides (e.g. β-1,4-d-(GlcNAc)6, CHI6), which involve several receptor kinase proteins (RKs) with LysM-ECDs. Here, we describe the isolation and characterization of Arabidopsis thaliana mutants impaired in glycan perception (igp) that are defective in PTI activation mediated by MLG43 and CEL3, but not by CHI6. igp1–igp4 are altered in three RKs – AT1G56145 (IGP1), AT1G56130 (IGP2/IGP3) and AT1G56140 (IGP4) – with leucine-rich-repeat (LRR) and malectin (MAL) domains in their ECDs. igp1 harbors point mutation E906K and igp2 and igp3 harbor point mutation G773E in their kinase domains, whereas igp4 is a T-DNA insertional loss-of-function mutant. Notably, isothermal titration calorimetry (ITC) assays with purified ECD-RKs of IGP1 and IGP3 showed that IGP1 binds with high affinity to CEL3 (with dissociation constant KD = 1.19 ± 0.03 μm) and cellopentaose (KD = 1.40 ± 0.01 μM), but not to MLG43, supporting its function as a plant PRR for cellulose-derived oligosaccharides. Our data suggest that these LRR-MAL RKs are components of a recognition mechanism for both cellulose- and MLG-derived oligosaccharide perception and downstream PTI activation in Arabidopsis.
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Keywords

beta-1,3 glucanscellulosedefense responsesdomainelicitorsimmunityleucine-reach repeatlinkagemalectin receptor kinase (lrr-mal rk)mixed-linked glucans (mlgs)oligosaccharidesoscerk1pattern recognition receptors (prrs)plantproteinrecognitionArabidopsisArabidopsis proteinsArabidopsis thalianaCelluloseGlucansImmunityLeucineLeucine-reach repeat/malectin receptor kinase (lrr-mal rk)Mixed-linked glucans (mlgs)Molecular-patternsOligosaccharidesPattern recognition receptors (prrs)Plant immunityPlantsProtein serine-threonine kinases

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal PLANT JOURNAL due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2023, it was in position 19/265, thus managing to position itself as a Q1 (Primer Cuartil), in the category Plant Sciences. Notably, the journal is positioned above the 90th percentile.

This publication has been distinguished as a “Highly Cited Paper” by the agencies WoS (ESI, Clarivate) and ESI (Clarivate), meaning that it ranks within the top 1% of the most cited articles in its thematic field during the year of its publication. In terms of the observed impact of the contribution, this work is considered one of the most influential worldwide, as it is recognized as highly cited. (source consulted: ESI Nov 13, 2025)

And this is evidenced by the extremely high normalized impacts through some of the main indicators of this type, which, although dynamic over time and dependent on the set of average global citations at the time of calculation, already indicate that they are well above the average in different agencies:

  • Normalization of citations relative to the expected citation rate (ESI) by the Clarivate agency: 9.13 (source consulted: ESI Nov 13, 2025)
  • Weighted Average of Normalized Impact by the Scopus agency: 8.85 (source consulted: FECYT Mar 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2026-04-28, the following number of citations:

  • WoS: 44
  • Scopus: 46
  • Google Scholar: 11
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-28:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 54.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 54 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 17.
  • The number of mentions on the social network X (formerly Twitter): 1 (Altmetric).
  • The number of mentions in news outlets: 2 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://oa.upm.es/92699/

As a result of the publication of the work in the institutional repository, statistical usage data has been obtained that reflects its impact. In terms of dissemination, we can state that, as of

  • Views: 35
  • Downloads: 29
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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Sweden; Switzerland; United States of America.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (MARTIN DACAL, MARINA) and Last Author (MOLINA FERNANDEZ, ANTONIO).

the authors responsible for correspondence tasks have been FERNANDEZ CALVO, PATRICIA and MOLINA FERNANDEZ, ANTONIO.

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