{rfName}
A

Indexed in

License and Use

Icono OpenAccess

Citations

8

Altmetrics

Analysis of institutional authors

Ballesteros-Gutiérrez M.Author or co-author of article in journal with external admissions assessment committeeAlbareda MAuthorPalacios JmCorresponding Author

Share

June 19, 2023
Publications
>
Article
Sí

A host-specific diaminobutyrate aminotransferase contributes to symbiotic performance, homoserine metabolism, and competitiveness in the Rhizobium leguminosarum/Pisum sativum system

Publicated to: Frontiers in Microbiology. 14 1182563- - 2023-05-16 14(), DOI: https://doi.org/10.3389/fmicb.2023.1182563

Authors:

Ballesteros-Gutiérrez, M; Albareda, M; Barbas, C; López-Gonzálvez, A; Dunn, MF; Palacios, JM
[+]

Affiliations

Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Programa Genom Func Procariotes, Cuernavaca, Mexico - Author
Univ Politecn Madrid, Ctr Biotecnol & Genomica Plantas CBGP, Consejo Super Invest Cient, Inst Nacl Invest & Tecnol Agr & Alimentaria,UPM,IN, Pozuelo de Alarcon, Spain - Author
Univ Politecn Madrid, Escuela Tecn Super Ingn Agron Alimentaria & Biosis, Dept Biotecnol Biol Vegetal, Madrid, Spain - Author
Univ San Pablo CEU, Fac Farm, Ctr Metabol & Bioanal CEMBIO, Boadilla del Monte, Spain - Author
Universidad Nacional Autónoma de México Campus Morelos - Author
Universidad Politécnica de Madrid - Author
Universidad San Pablo-CEU - Author
See more

Abstract

Rhizobium leguminosarum bv. viciae (Rlv) UPM791 effectively nodulates pea and lentil, but bacteroids contain a number of proteins differentially expressed depending on the host. One of these host-dependent proteins (C189) is similar to a diaminobutyrate-2-oxoglutarate aminotransferase (DABA-AT). DABA-AT activity was demonstrated with cell extracts and with purified protein, so C189 was renamed as Dat. The dat gene was strongly induced in the central, active area of pea nodules, but not in lentil. Mutants defective in dat were impaired in symbiotic performance with pea plants, exhibiting reduced shoot dry weight, smaller nodules, and a lower competitiveness for nodulation. In contrast, there were no significant differences between mutant and wild-type in symbiosis with lentil plants. A comparative metabolomic approach using cell-free extracts from bacteroids induced in pea and lentil showed significant differences among the strains in pea bacteroids whereas no significant differences were found in lentil. Targeted metabolomic analysis revealed that the dat mutation abolished the presence of 2,4-diaminobutyrate (DABA) in pea nodules, indicating that DABA-AT reaction is oriented toward the production of DABA from L-aspartate semialdehyde. This analysis also showed the presence of L-homoserine, a likely source of aspartate semialdehyde, in pea bacteroids but not in those induced in lentil. The dat mutant showed impaired growth when cells were grown with L-homoserine as nitrogen source. Inclusion of DABA or L-homoserine as N source suppressed pantothenate auxotropy in Rlv UPM791, suggesting DABA as source of the pantothenate precursor β-alanine. These data indicate that Rlv UPM791 Dat enzyme is part of an adaptation mechanism of this bacterium to a homoserine-rich environment such as pea nodule and rhizosphere.
[+]

Keywords

alanine dehydrogenasebacteroidcloningectoineescherichia-coliexpressiongenesnodulationnodule developmentpantothenate auxotrophypolyaminessinorhizobium-melilotisymbiosisBacteroidBv viciaeNitrogen fixationNodulationPantothenate auxotrophySymbiosis

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Frontiers in Microbiology due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2023, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Microbiology (Medical).

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2026-04-25:

  • WoS: 4
  • Scopus: 4
[+]

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-25:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 14.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 13 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 4.
  • The number of mentions on the social network X (formerly Twitter): 6 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://oa.upm.es/92299/

As a result of the publication of the work in the institutional repository, statistical usage data has been obtained that reflects its impact. In terms of dissemination, we can state that, as of

  • Views: 41
  • Downloads: 22
[+]

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Mexico.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (BALLESTEROS GUTIERREZ, MARTA) and Last Author (PALACIOS ALBERTI, JOSE MANUEL).

the author responsible for correspondence tasks has been PALACIOS ALBERTI, JOSE MANUEL.

[+]