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Analysis of institutional authors

Sáez C, Kheireddine A, García A, Sifres A, Moreno A, Font-San-Ambrosio Mi, Picó B, López CCorresponding Author

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November 27, 2023
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Further Molecular Diagnosis Determines Lack of Evidence for Real Seed Transmission of Tomato Leaf Curl New Delhi Virus in Cucurbits

Publicated to: Plants-Basel. 12 (21): 3773- - 2023-11-01 12(21), DOI: 10.3390/plants12213773

Authors:

Sáez, C; Kheireddine, A; Garcia, A; Sifres, A; Moreno, A; Font-San-Ambrosio, MI; Picó, B; López, C
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Affiliations

Consejo Superior de Investigaciones Científicas - Author
Sakata Vegetables Europe - Author
Sakata Vegetables Europe Ltd, Almeria 04738, Spain - Author
Univ Politecn Madrid, Ctr Biotecnol & Genomica Plantas, UPM INIA & ETS Ingn Agron Alimentaria & Biosistema, Madrid 28031, Spain - Author
Univ Politecn Valencia COMAV UPV, Inst Conservat & Breeding Valencian Agrodivers, Camino Vera S-N, Valencia 46022, Spain - Author
Univ Politecn Valencia IAM UPV, Inst Agroforestal Mediterraneo, Camino Vera S-N, Valencia 46022, Spain - Author
Univ Politecn Valencia, Consejo Super Invest Cient, Inst Biol Mol & Celular Plantas, Camino Vera S-N, Valencia 46022, Spain - Author
Universidad Politécnica de Madrid - Author
Universidad Politécnica de Madrid , Universitat Politècnica de València - Author
Universitat Politècnica de València - Author
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Abstract

Begomoviruses (family Geminiviridae) cause serious diseases in many crop families. Since 2013, the Spanish isolate of tomato leaf curl New Delhi virus (ToLCNDV) has been a limiting factor for cucurbits production in the Mediterranean basin, forcing farmers to adapt new management and control techniques. Although it is well-known that begomoviruses are naturally transmitted by the whitefly Bemisia tabaci, the capacity of these viruses to be vertically transmitted through seeds remains controversial. Clarifying the potential ToLCNDV seed transmission is essential to understand the epidemiology of this threating-for-cucurbits virus and to design appropriate control strategies. We assessed ToLCNDV distribution in the leaves, flowers and seeds of the infected plants of susceptible Cucumis melo accessions and toleration to the infected genotypes of Cucurbita moschata by conventional and quantitative PCR. We analyzed whether the viral particle was transmitted to offspring. We also evaluated ToLCNDV presence in commercial seeds of cucurbits (zucchini (Cucurbita pepo), melon (C. melo), cucumber (Cucumis sativus) and watermelon (Citrullus lanatus)) and in their progenies. As the assayed seedlings remained symptomless, we increased the reliability and accuracy of detection in these samples by searching for replicative forms of ToLCNDV by combining Southern blot hybridization and rolling-circle amplification (RCA). However, integral genomic DNA was not identified in the plants of offspring. Although the seedborne nature of ToLCNDV was confirmed, our results do not support the transmission of this virus from contaminated seeds to progeny.
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Keywords

1st reportbegomoviruscucurbit seedlingsevolutiongenomeindiapotatoqpcrrolling-circle amplificationtolcndvBegomovirusCucurbit seedlingsInfecting zucchiniQpcrRolling-circle amplificationSeed transmissionTolcndv

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Plants-Basel due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2023, it was in position 46/265, thus managing to position itself as a Q1 (Primer Cuartil), in the category Plant Sciences.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2026-04-27:

  • WoS: 3
  • Scopus: 3
  • Europe PMC: 1
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-27:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 15.

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 2.
  • The number of mentions on the social network X (formerly Twitter): 2 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://oa.upm.es/82363/

As a result of the publication of the work in the institutional repository, statistical usage data has been obtained that reflects its impact. In terms of dissemination, we can state that, as of

  • Views: 237
  • Downloads: 34
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Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (SAEZ SANCHEZ, CRISTINA) .

the author responsible for correspondence tasks has been SAEZ SANCHEZ, CRISTINA.

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