{rfName}
Co

Indexed in

License and use

Citations

2

Altmetrics

Article has an altmetric score of 2

Analysis of institutional authors

Rodriguez Romero, Julio LuisAuthorRuiz Padilla, AnaAuthorAyllon Talavera, Maria AngelesAuthorPardo Medina, JavierAuthorCórdoba L, Ruiz-Padilla A, Pardo-Medina J, Rodríguez-Romero Jl, Ayllón MaAuthor

Share

February 5, 2024
Publications
>
Article
No

Construction of a Mycoviral Infectious Clone for Reverse Genetics in Botrytis cinerea

Publicated to:Methods In Molecular Biology. 2751 47-68 - 2024-01-01 2751(), DOI: 10.1007/978-1-0716-3617-6_4

Authors: Córdoba L; Ruiz-Padilla A; Pardo-Medina J; Rodríguez-Romero JL; Ayllón MA

Affiliations

CSIC-UPM-INIA-CBGP - Centro de Biotecnología y Genómica de Plantas - Author
CSIC-UPM-INIA-CBGP - Centro de Biotecnología y Genómica de Plantas , Universidad de Sevilla - Author
CSIC-UPM-INIA-CBGP - Centro de Biotecnología y Genómica de Plantas , Universidad Politécnica de Madrid - Author
Universidad de Sevilla - Author
Universidad Politécnica de Madrid - Author
See more

Abstract

The most important advances in our understanding of the viral life cycle, such as genome replication, packaging, transmission, and host interactions, have been made via the development of viral infectious full-length clones. Here, we describe the detailed protocols for the construction of an infectious clone derived from Botrytis virus F (BVF), a mycoflexivirus infecting the plant pathogenic fungus Botrytis cinerea, the determination of the complete sequence of the cloned mycovirus, the preparation of fungal protoplasts, and the transfection of protoplasts using transcripts derived from the BVF infectious clone.

Keywords

BotrytisBotrytis cinereaCommunicable diseasesFungal virusesFungusMycovirusReverse geneticsSynthetic virusViral infectious cloneVirus

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Methods In Molecular Biology, Q3 Agency Scopus (SJR), its regional focus and specialization in Genetics, give it significant recognition in a specific niche of scientific knowledge at an international level.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-07-08:

  • Scopus: 1
  • Europe PMC: 1

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-07-08:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 2.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 2 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 1.75.
  • The number of mentions on the social network Facebook: 1 (Altmetric).
  • The number of mentions on the social network X (formerly Twitter): 2 (Altmetric).

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (CORDOBA GARCIA, LAURA) .