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Analysis of institutional authors
Rodriguez Romero, Julio LuisAuthorRuiz Padilla, AnaAuthorAyllon Talavera, Maria AngelesAuthorPardo Medina, JavierAuthorCórdoba L, Ruiz-Padilla A, Pardo-Medina J, Rodríguez-Romero Jl, Ayllón MaAuthorConstruction of a Mycoviral Infectious Clone for Reverse Genetics in Botrytis cinerea
Publicated to:Methods In Molecular Biology. 2751 47-68 - 2024-01-01 2751(), DOI: 10.1007/978-1-0716-3617-6_4
Authors: Córdoba L; Ruiz-Padilla A; Pardo-Medina J; Rodríguez-Romero JL; Ayllón MA
Affiliations
Abstract
The most important advances in our understanding of the viral life cycle, such as genome replication, packaging, transmission, and host interactions, have been made via the development of viral infectious full-length clones. Here, we describe the detailed protocols for the construction of an infectious clone derived from Botrytis virus F (BVF), a mycoflexivirus infecting the plant pathogenic fungus Botrytis cinerea, the determination of the complete sequence of the cloned mycovirus, the preparation of fungal protoplasts, and the transfection of protoplasts using transcripts derived from the BVF infectious clone.
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Bibliometric impact. Analysis of the contribution and dissemination channel
The work has been published in the journal Methods In Molecular Biology, Q3 Agency Scopus (SJR), its regional focus and specialization in Genetics, give it significant recognition in a specific niche of scientific knowledge at an international level.
Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.
Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-07-08:
- Scopus: 1
- Europe PMC: 1
Impact and social visibility
Leadership analysis of institutional authors
There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (CORDOBA GARCIA, LAURA) .