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Analysis of institutional authors

Corregidor-Ortiz, DanielAuthorDaza, RafaelAuthorColchero, LuisAuthorTabraue-Rubio, RaquelAuthorAtienza, Jose MiguelAuthorElices, ManuelAuthorGuinea, Gustavo VAuthorPerez-Rigueiro, JoseCorresponding Author

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May 13, 2024
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Identification of Individual Target Molecules Using Antibody-Decorated DeepTip™ Atomic-Force Microscopy Probes

Publicated to: Biomimetics (Basel). 9 (4): 192- - 2024-04-01 9(4), DOI: 10.3390/biomimetics9040192

Authors:

Corregidor-Ortiz, D; Daza, R; Colchero, L; Tabraue-Rubio, R; Atienza, JM; Elices, M; Guinea, GV; Pérez-Rigueiro, J
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Affiliations

Bioact Srfaces SL, C Puerto Navacerrada 18, Galapagar 28260, Spain - Author
Biomed Res Networking Ctr Bioengn Biomat & Nanomed, Madrid 28029, Spain - Author
Inst Invest Sanitaria Hosp Clin San Carlos IdISSC, Biomat & Regenerat Med Grp, C Prof Martin Lagos S-N, Madrid 28040, Spain - Author
Univ Politecn Madrid, Ctr Biomed Technol CTB, Madrid 28223, Spain - Author
Univ Politecn Madrid, ETSI Caminos Canales & Puertos, Dept Ciencia Mat, Madrid 28040, Spain - Author
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Abstract

A versatile and robust procedure is developed that allows the identification of individual target molecules using antibodies bound to a DeepTip (TM) functionalized atomic-force microscopy probe. The model system used for the validation of this process consists of a biotinylated anti-lactate dehydrogenase antibody immobilized on a streptavidin-decorated AFM probe. Lactate dehydrogenase (LDH) is employed as target molecule and covalently immobilized on functionalized MicroDeck (TM) substrates. The interaction between sensor and target molecules is explored by recording force-displacement (F-z) curves with an atomic-force microscope. F-z curves that correspond to the genuine sensor-target molecule interaction are identified based on the following three criteria: (i) number of peaks, (ii) value of the adhesion force, and (iii) presence or absence of the elastomeric trait. The application of these criteria leads to establishing seven groups, ranging from no interaction to multiple sensor-target molecule interactions, for which force-displacement curves are classified. The possibility of recording consistently single-molecule interaction events between an antibody and its specific antigen, in combination with the high proportion of successful interaction events obtained, increases remarkably the possibilities offered by affinity atomic-force microscopy for the characterization of biological and biomimetic systems from the molecular to the tissue scales.
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Keywords

Affinity atomic-force microscopyAntibodyAntigenDnaFunctionalizationSingle-molecule resolution

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Biomimetics (Basel) due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 31/179, thus managing to position itself as a Q1 (Primer Cuartil), in the category Engineering, Multidisciplinary.

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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-27:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 7.

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 1.
  • The number of mentions on the social network X (formerly Twitter): 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://oa.upm.es/90216/

As a result of the publication of the work in the institutional repository, statistical usage data has been obtained that reflects its impact. In terms of dissemination, we can state that, as of

  • Views: 86
  • Downloads: 22
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Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (CORREGIDOR ORTIZ, DANIEL) and Last Author (PEREZ RIGUEIRO, JOSE).

the author responsible for correspondence tasks has been PEREZ RIGUEIRO, JOSE.

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Awards linked to the item

No Statement Available
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