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Analysis of institutional authors

Rincon, SaraAuthorZurita, LuciaAuthorPonz, FernandoCorresponding Author

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June 2, 2024
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Article

Antigen-functionalized turnip mosaic virus nanoparticles increase antibody sensing in saliva. A case study with SARS-CoV-2 RBD

Publicated to: Diagnostic Microbiology And Infectious Disease. 109 (3): 116298- - 2024-07-01 109(3), DOI: 10.1016/j.diagmicrobio.2024.116298

Authors:

Medrano-Arranz, C; Rincón, S; Zurita, L; Ponz, F; Truchado, DA
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Affiliations

Univ Politecn Madrid UPM, Ctr Biotecnol & Genom Plantas, Inst Nacl Invest Agr & Alimentaria INIA CSIC, Campus Montegancedo UPM, Pozuelo De Alarcon 28223, Madrid, Spain - Author

Abstract

Nanoparticles derived from plant viruses play an important role in nanomedicine due to their biocompatibility, self-assembly and easily-modifiable surface. In this study, we developed a novel platform for increasing antibody sensing using viral nanoparticles derived from turnip mosaic virus (TuMV) functionalized with SARS-CoV-2 receptor binding domain (RBD) through three different methods: chemical conjugation, gene fusion and the SpyTag/SpyCatcher technology. Even though gene fusion turned out to be unsuccessful, the other two constructs were proven to significantly increase antibody sensing when tested with saliva of patients with different infection and vaccination status to SARS-CoV-2. Our findings show the high potential of TuMV nanoparticles in the fields of diagnostics and immunodetection, being especially attractive for the development of novel antibody sensing devices .
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Keywords

Antibodies, viralAntibody sensingAntigens, viralCovid-19HumansIgM13 bacteriophageNanoparticlesSalivaSalivaigaSalivaigaSars-cov-2Spike glycoprotein, coronavirusTurnip mosaic virusTymovirusViral nanoparticlesVlp

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Diagnostic Microbiology And Infectious Disease due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position , thus managing to position itself as a Q2 (Segundo Cuartil), in the category Medicine (Miscellaneous). Notably, the journal is positioned en el Cuartil Q3 for the agency WoS (JCR) in the category Infectious Diseases.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-12-21:

  • WoS: 1
  • Scopus: 1
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-12-21:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 7.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 7 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 1.
  • The number of mentions on the social network X (formerly Twitter): 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
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Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Medrano-Arranz, Carlos) and Last Author (Truchado, Daniel A).

the author responsible for correspondence tasks has been Ponz Ascaso, Fernando.

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Awards linked to the item

This work was mostly funded by grant COV20/00114 from Comunidad de Madrid to FP. We want to particularly acknowledge the donors and the Biobank Biobanco Hospital Universitario de La Princesa (Madrid) (ISCIII B.0000763) for their collaboration. We also thank Agrenvec S.L (Tres Cantos, Madrid, Spain) for the generous gift of its plant -made RBD. DAT was supported by a Margarita Salas postdoctoral grant funded by the Spanish Ministry of Universities and UCM (CT31/21) . SR was funded by a contract under grant P2018/BAA-4574 from the Comunidad de Madrid. This work was part of the Final Grade Thesis of CM for graduation in biotechnology by the UPM. The CBGP was granted 'Severo Ochoa' Distinctions of Excellence by the Spanish Ministry of Science and Innovation (SEV- 2016-0672 and CEX2020-000999-S) .
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