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Analysis of institutional authors

Rodríguez-Dobreva E.Author

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June 3, 2024
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A new oxidative pathway of nitric oxide production from oximes in plants

Publicated to: Molecular Plant. 17 (1): 178-198 - 2024-01-01 17(1), DOI: 10.1016/j.molp.2023.12.009

Authors:

López-Gómez P; Buezo J; Urra M; Cornejo A; Esteban R; Fernández de los Reyes J; Urarte E; Rodríguez-Dobreva E; Chamizo-Ampudia A; Eguaras A; Wolf S; Marino D; Martínez-Merino V; Moran JF
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Affiliations

Center for Plant Molecular Biology (ZMBP); University of Tübingen; Geschwister-Scholl-Platz; Tübingen; 72074; Germany - Author
Department of Plant Biology and Ecology; University of the Basque Country (UPV/EHU); Sarriena s/n; Apdo. 644; Bilbao; 48080; Spain - Author
Institute for Advanced Materials and Mathematics (INAMAT2); Department of Sciences; Public University of Navarre (UPNA); Campus de Arrosadía; Pamplona; 31006; Spain - Author
Institute for Multidisciplinary Research in Applied Biology (IMAB); Department of Sciences; Public University of Navarre (UPNA); Avda. de Pamplona 123; Mutilva; 31192; Spain - Author
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Abstract

Nitric oxide (NO) is an essential reactive oxygen species and a signal molecule in plants. Although several studies have proposed the occurrence of oxidative NO production, only reductive routes for NO production, such as the nitrate (NO-3) -upper-reductase pathway, have been evidenced to date in land plants. However, plants grown axenically with ammonium as the sole source of nitrogen exhibit contents of nitrite and NO3−, evidencing the existence of a metabolic pathway for oxidative production of NO. We hypothesized that oximes, such as indole-3-acetaldoxime (IAOx), a precursor to indole-3-acetic acid, are intermediate oxidation products in NO synthesis. We detected the production of NO from IAOx and other oximes catalyzed by peroxidase (POD) enzyme using both 4-amino-5-methylamino-2′,7′-difluorescein fluorescence and chemiluminescence. Flavins stimulated the reaction, while superoxide dismutase inhibited it. Interestingly, mouse NO synthase can also use IAOx to produce NO at a lower rate than POD. We provided a full mechanism for POD-dependent NO production from IAOx consistent with the experimental data and supported by density functional theory calculations. We showed that the addition of IAOx to extracts from Medicago truncatula increased the in vitro production of NO, while in vivo supplementation of IAOx and other oximes increased the number of lateral roots, as shown for NO donors, and a more than 10-fold increase in IAOx dehydratase expression. Furthermore, we found that in vivo supplementation of IAOx increased NO production in Arabidopsis thaliana wild-type plants, while prx33-34 mutant plants, defective in POD33-34, had reduced production. Our data show that the release of NO by IAOx, as well as its auxinic effect, explain the superroot phenotype. Collectively, our study reveals that plants produce NO utilizing diverse molecules such as oximes, POD, and flavins, which are widely distributed in the plant kingdom, thus introducing a long-awaited oxidative pathway to NO production in plants. This knowledge has essential implications for understanding signaling in biological systems. © 2023 The Author
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Keywords

Ammonium oxidationAnimalAnimalsArabidopsisAuxinFlavinsMetabolismMiceMouseNitric oxideOxidative stressOximeOximesPeroxidaseRiboflavin derivativeRoot phenotype

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Molecular Plant due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 2/273, thus managing to position itself as a Q1 (Primer Cuartil), in the category Plant Sciences. Notably, the journal is positioned above the 90th percentile.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-12-25:

  • Scopus: 15
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-12-25:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 23.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 29 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 43.
  • The number of mentions on the social network X (formerly Twitter): 31 (Altmetric).
  • The number of mentions in news outlets: 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Germany.

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