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Impact on the Sustainable Development Goals (SDGs)

Analysis of institutional authors

Munar-Palmer, MartiAuthorSantamaria-Hernando, SarayAuthorLopez-Torrejon, GemaAuthorRodriguez-Herva, Jose JuanAuthorLopez-Solanilla, EmiliaCorresponding Author

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July 2, 2024
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Article

Chemosensory systems interact to shape relevant traits for bacterial plant pathogenesis

Publicated to: mBio. 15 (7): - 2024-07-17 15(7), DOI: 10.1128/mbio.00871-24

Authors:

Munar-Palmer, Marti; Santamaria-Hernando, Saray; Liedtke, Janine; Ortega, Davi R; Ortega, Davi R; Lopez-Torrejon, Gema; Rodriguez-Herva, Jose Juan; Briegel, Ariane; Lopez-Solanilla, Emilia
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Affiliations

Leiden Univ, Inst Biol, Leiden, Netherlands - Author
Univ Politecn Madrid UPM, Ctr Biotecnol & Genomica Plantas, Inst Nacl Invest & Tecnol Agr & Alimentaria INIA C, Madrid, Spain - Author
Univ Politecn Madrid UPM, Escuela Tecn Super Ingn Agron, Dept Biotecnol Biol Vegetal, Madrid, Spain - Author
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Abstract

Chemosensory systems allow bacteria to respond and adapt to environmental conditions. Many bacteria contain more than one chemosensory system, but knowledge of their specific roles in regulating different functions remains scarce. Here, we address this issue by analyzing the function of the F6, F8, and alternative (non-motility) cellular functions (ACF) chemosensory systems of the model plant pathogen Pseudomonas syringae pv. tomato. In this work, we assign PsPto chemoreceptors to each chemosensory system, and we visualize for the first time the F6 and F8 chemosensory systems of PsPto using cryo-electron tomography. We confirm that chemotaxis and swimming motility are controlled by the F6 system, and we demonstrate how different components from the F8 and ACF systems also modulate swimming motility. We also determine how the kinase and response regulators from the F6 and F8 chemosensory systems do not work together in the regulation of biofilm, whereas both components from the ACF system contribute together to regulate these traits. Furthermore, we show how the F6, F8, and ACF kinases interact with the ACF response regulator WspR, supporting crosstalk among chemosensory systems. Finally, we reveal how all chemosensory systems play a role in regulating virulence.
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Keywords

BiofilmBiofilm formationC-di-gmpChemoperceptionChemotaxisCrosstalkLife on landMotilityPathwayProteinPseudomonas syringaePseudomonas-aeruginosaPv. tomato dc3000Signal-transductionTwitching motilityVirulencVirulence

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal mBio due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 33/163, thus managing to position itself as a Q1 (Primer Cuartil), in the category Microbiology.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2026-04-09:

  • WoS: 4
  • Scopus: 3
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-09:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 9.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 9 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 7.
  • The number of mentions on the social network X (formerly Twitter): 12 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://oa.upm.es/86207/

As a result of the publication of the work in the institutional repository, statistical usage data has been obtained that reflects its impact. In terms of dissemination, we can state that, as of

  • Views: 110
  • Downloads: 50
Continuing with the social impact of the work, it is important to emphasize that, due to its content, it can be assigned to the area of interest of ODS 15 - Life on land, with a probability of 41% according to the mBERT algorithm developed by Aurora University.
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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Netherlands.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (MUNAR PALMER, MARTI) and Last Author (LOPEZ SOLANILLA, EMILIA ANTONIA).

the author responsible for correspondence tasks has been LOPEZ SOLANILLA, EMILIA ANTONIA.

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Project objectives

El presente estudio persigue los siguientes objetivos: analizar la función de los sistemas quimiosensoriales F6, F8 y ACF en Pseudomonas syringae pv. tomato; asignar los quimiorreceptores PsPto a cada sistema quimiosensorial; visualizar mediante criotomografía electrónica los sistemas F6 y F8; determinar el control de la quimiotaxis y motilidad nadadora por el sistema F6 y la modulación de esta motilidad por componentes de los sistemas F8 y ACF; evaluar la regulación conjunta o independiente de biofilm por quinasas y reguladores de respuesta de los sistemas F6, F8 y ACF; y caracterizar las interacciones entre quinasas y reguladores que evidencian comunicación cruzada entre sistemas, así como su papel en la regulación de la virulencia bacteriana.
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Most relevant results

El estudio analiza la función de los sistemas quimiosensoriales F6, F8 y ACF en Pseudomonas syringae pv. tomato, modelo de patógeno vegetal. Se asignaron receptores quimiosensoriales a cada sistema y se visualizaron por primera vez los sistemas F6 y F8 mediante criotomografía electrónica. Se confirmó que el sistema F6 controla la quimiotaxis y la motilidad nadadora, mientras que los sistemas F8 y ACF también modulan esta motilidad. Se determinó que las quinasas y reguladores de F6 y F8 no cooperan en la regulación del biofilm, pero los componentes de ACF sí contribuyen conjuntamente. Además, se evidenció la interacción entre quinasas F6, F8 y ACF con el regulador WspR, indicando comunicación entre sistemas. Todos los sistemas regulan la virulencia bacteriana.
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Awards linked to the item

We acknowledge Christopher R. Clarke for kindly providing the construct pME6010::CheA2 and Sandra Nebreda for technical assistance.
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