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Analysis of institutional authors

Sanchez-Maroto, LauraAuthorGella, PabloAuthorCouce, AlejandroCorresponding Author

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December 3, 2024
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Novel Fosfomycin Resistance Mechanism in Pseudomonas entomophila Due to Atypical Pho Regulon Control of GlpT

Publicated to: Antibiotics. 13 (11): 1008- - 2024-11-01 13(11), DOI: 10.3390/antibiotics13111008

Authors:

Sánchez-Maroto, L; Gella, P; Couce, A
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Affiliations

Univ Politecn Madrid UPM, Ctr Biotecnol & Genom Plantas CBGP, Madrid 28223, Spain - Author

Abstract

Background/Objectives: Pseudomonas entomophila is a ubiquitous bacterium capable of killing insects of different orders and has become a model for host-pathogen studies and a promising tool for biological pest control. In the human pathogen Pseudomonas aeruginosa, spontaneous resistance to fosfomycin arises almost exclusively from mutations in the glycerol-3-phosphate transporter (GlpT), the drug's sole entry route in this species. Here, we investigated whether this specificity is conserved in P. entomophila, as it could provide a valuable marker system for studying mutation rates and spectra and for selection in genetic engineering. Methods: We isolated 16 independent spontaneous fosfomycin-resistant mutants in P. entomophila, and studied the genetic basis of the resistance using a combination of sequencing, phenotyping and computational approaches. Results: We only found two mutants without alterations in glpT or any of its known regulatory elements. Whole-genome sequencing revealed unique inactivating mutations in phoU, a key regulator of the phosphate starvation (Pho) regulon. Computational analyses identified a PhoB binding site in the glpT promoter, and experiments showed that phoU inactivation reduced glpT expression nearly 20-fold. While placing a sugar-phosphate transporter under the Pho regulon may seem advantageous, bioinformatic analysis shows this configuration is atypical among pseudomonads. Conclusions: This atypical Pho regulon control of GlpT probably reflects the peculiarities of P. entomophila's habitat and lifestyle; highlighting how readily regulatory evolution can lead to the rapid divergence of resistance mechanisms, even among closely related species.
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Keywords

Fosfomycin resistanceGeneGlptIdentificationInfectionMutation ratePho regulonPhosphatePromoter evolutioPromoter evolutionRepressorSysteTranscriptionUrinary-tract

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Antibiotics due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 17/137, thus managing to position itself as a Q1 (Primer Cuartil), in the category Infectious Diseases.

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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-26:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 4.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 4 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 1.

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://oa.upm.es/89084/

As a result of the publication of the work in the institutional repository, statistical usage data has been obtained that reflects its impact. In terms of dissemination, we can state that, as of

  • Views: 104
  • Downloads: 26
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Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (SANCHEZ-MAROTO CAPILLA, LAURA) and Last Author (COUCE IGLESIAS, ALEJANDRO).

the author responsible for correspondence tasks has been COUCE IGLESIAS, ALEJANDRO.

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Awards linked to the item

This research was funded by the Agencia Estatal de Investigacion (Proyectos de I + D + i, PID2019-110992GA-I00; Centros de Excelencia "Severo Ochoa", SEV-2016-0672 and CEX2020-000999-S; FPI PhD studentship, PRE2018-086448), and a Comunidad de Madrid "Talento" Fellowship (2019-T1/BIO-12882 and 2023-5A/BIO-28940).
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