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Analysis of institutional authors

Perez, CesarAuthor

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January 29, 2025
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Developing a Cryopreservation Protocol for Embryonic Axes of Six South American Peanut Genotypes (Arachis hypogaea L.) Using Desiccation-Vitrification

Publicated to: Crops. 4 (4): 701-716 - 2024-12-01 4(4), DOI: 10.3390/crops4040048

Authors:

Tacán, M; Andrango, R; Tapia, C; Sorensen, M; Vollmer, R; Pérez, C
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Affiliations

Cryobank, Int Potato Ctr CIP, Ave 1895 Molina, Lima 15023, Peru - Author
INIAP, Estn Expt Santa Catalina, Panamer Sur Km 1, Quito 171107, Ecuador - Author
Univ Copenhagen, Fac Sci, Dept Plant & Environm Sci, Sobi Plen KU,Thorvaldsensvej 40,3, DK-1871 Frederiksberg, Denmark - Author
Univ Politecn Madrid, Dept Biotecnol & Biol Vegetal, ETS Ingn Agron Alimentaria & Biosistemas, Madrid 28000, Spain - Author
Univ Rey Juan Carlos, Programa Doctorado Conservac Recursos Nat, Madrid 28933, Spain - Author
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Abstract

The present study investigates the cryopreservation of embryonic axes from the peanut genotype ECU-12466, demonstrating a successful protocol involving a 1 h desiccation on silica gel followed by a 1 h cryoprotection with Plant Vitrificatin Solution 2 (PVS2). The average dimensions of the excised embryonic axes were 5.6 mm in length and 3.5 mm in width, with plumule lengths averaging 2.2 mm. Notably, germination rates for cryopreserved axes ranged from 71.4% to 85.7%, showing resilience to varying desiccation and PVS2 treatment times, particularly at 1 h. Shoot length was significantly enhanced by a 1 h PVS2 exposure, while longer durations resulted in phytotoxic effects. Rooting rates were higher for samples treated with shorter desiccation periods, with 54% rooting achieved at 1 h of PVS2 exposure, contrasting sharply with just 16% at 2 h. The moisture content of the embryonic axes remained stable between 9.3% and 9.5%, indicating no detrimental impact from the applied treatments. To evaluate the protocol's broader applicability, five additional peanut genotypes (ECU-11401, ECU-11418, ECU-11448, ECU-11469, and ECU-11494) were tested. While cryopreserved samples demonstrated high germination rates of up to 95.4% after 15 days, the rooting success was significantly lower (25.2%) compared to the control group's 90.9%. Following transplantation, the growth performance varied among genotypes, with a success rate of 93% for ECU-11494. Overall, this study elucidates the critical parameters for optimizing cryopreservation protocols in peanut embryonic axes, contributing to more effective long-term conservation strategies.
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Keywords

ConservationCryoconservationDehydrationGermplasmGroundnutGrown shoot tipsPeanutPvsPvs2SeedSilica gelSomatic embryosSurvivalToleranc

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Crops due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 48/129, thus managing to position itself as a Q2 (Segundo Cuartil), in the category Agronomy. Notably, the journal is positioned en el Cuartil Q2 para la agencia Scopus (SJR) en la categoría .

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-12-21:

  • Scopus: 1
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-12-21:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 5.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 6 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

    It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

    • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
    • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://oa.upm.es/87471/

    As a result of the publication of the work in the institutional repository, statistical usage data has been obtained that reflects its impact. In terms of dissemination, we can state that, as of

    • Views: 115
    • Downloads: 24
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    Leadership analysis of institutional authors

    This work has been carried out with international collaboration, specifically with researchers from: Denmark; Ecuador; Peru.

    There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: Last Author (PEREZ RUIZ, CESAR FELIX).

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    Awards linked to the item

    This research was funded by the Research Fund for Agrobiodiversity, Seeds, and Sustainable Agriculture (FIASA) in Ecuador. On 16 January 2024, at a meeting of the FIASA Project Evaluation Committee, the project "Conservation and management of the INIAP Germplasm Bank (FIASA-EESC-2024-022)" was approved and allocated a budget.
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