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Analysis of institutional authors

Souiade, LiliaAuthorSerrano-Olmedo, Jose-JavierAuthorRamos-Gomez, MilagrosCorresponding Author

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April 22, 2025
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Pulsed Alternating Fields Magnetic Hyperthermia in Combination with Chemotherapy (5-Fluorouracil) as a Cancer Treatment for Glioblastoma Multiform: An In Vitro Study

Publicated to: Nanomaterials. 15 (7): 556- - 2025-04-05 15(7), DOI: 10.3390/nano15070556

Authors:

Souiade, L; Rodriguez-Garcia, MR; Serrano-Olmedo, JJ; Ramos-Gómez, M
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Affiliations

Inst Salud Carlos III, Ctr Invest Biomed Red Bioingn Biomat & Nanomed, Madrid 28029, Spain - Author
Univ Politecn Madrid, Ctr Tecnol Biomed, Campus Montegancedo, Pozuelo De Alarcon 28223, Madrid, Spain - Author
Univ Politecn Madrid, Dept Tecnol Foton & Bioingn, ETSI Telecomunicac, Madrid 28040, Spain - Author
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Abstract

Inducing magnetic hyperthermia (MHT) involves locally raising the temperature to 39-45 degrees C, which increases the susceptibility of tumor cells to therapeutic agents without damaging healthy tissues. Recent studies on trapezoidal pulsed alternating magnetic fields (TP-AMFs) have proven their considerable efficacy in increasing the temperature of magnetic nanoparticles (MNPs) compared to sinusoidal fields. Thermal therapies have been known to incorporate multiple combinations of therapeutic approaches to optimize the medical procedure for healing cancer patients such as chemotherapy and radiotherapy. The combination of MHT with chemotherapy aims to enhance the therapeutic effects against cancer due to the synergistic interaction in tumor cells. In this study, we aim to exploit the synergistic effects of combining MHT produced by TP-AMFs with a low concentration of 5-Fluorouracil (5-FU) to optimize the therapeutic outcomes in comparison to TP-AMFs MHT alone. Hence, we exposed a glioblastoma cell line (CT2A) incubated with iron oxide nanoparticles at 1 mg/mL to two cycles of MHT employing a trapezoidal-square waveform at 200 kHz and 2 mT for 30 min for each cycle, separated by a 45 min break, both as a single treatment and in combination with 0.1 mu g/mL of 5-FU. Our findings demonstrated the efficacy of the synergistic effect between MHT treatment via TP-AMFs and the 5-FU, increasing the cell death to 58.9 +/- 2%, compared to 31.4 +/- 3% with MHT treatment alone. Cell death was primarily driven by the necrosis pathway (47.3 +/- 2%) compared to apoptosis (11.6 +/- 2). The addition of 5-FU enhanced the cytotoxic effect of MHT on CT2A cells, increasing the calreticulin (CRT) positive cells to 17 +/- 1% compared to 10 +/- 1% as produced by MHT treatment alone. Furthermore, this combination suggests that the employed treatment approach can promote immune system activation due to the exposure of CRT in the treated cells.
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Keywords

CancerChemotherapyCrtIcIcdMagnetic hyperthermiaNanomedicineNanoparticlesPulsed amfs

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Nanomaterials due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2025, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Chemical Engineering (Miscellaneous).

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2026-04-25:

  • WoS: 1
  • Scopus: 1
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-25:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 19.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 18 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 1.
  • The number of mentions on the social network X (formerly Twitter): 3 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://oa.upm.es/95100/

As a result of the publication of the work in the institutional repository, statistical usage data has been obtained that reflects its impact. In terms of dissemination, we can state that, as of

  • Views: 13
  • Downloads: 6
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Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (SOUIADE, LILIA) and Last Author (RAMOS GOMEZ, MARIA MILAGROS).

the author responsible for correspondence tasks has been RAMOS GOMEZ, MARIA MILAGROS.

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Awards linked to the item

The PhD scholarship was fully financed by the Algerian Ministry of Higher Education and Scientific Research. This study was partially funded by the Ministerio de Ciencia, Innovacion y Universidades of Spain, ref.: PGC2018-097531-B-I00 and PDC2022-133028-I00, funded by the European Union-NextGenerationEU.
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