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Analysis of institutional authors

Benito Casado, BegoÑaAuthorBenito, BAuthorRodriguez-Navarro, ACorresponding Author

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June 9, 2019
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Novel P-type ATPases mediate high-affinity potassium or sodium uptake in fungi

Publicated to:Eukaryotic Cell. 3 (2): 359-368 - 2004-04-01 3(2), DOI: 10.1128/EC.3.2.359-368.2004

Authors: Benito, B; Garciadeblás, B; Schreier, P; Rodríguez-Navarro, A

Affiliations

Bayer CropSci R TRF, D-40789 Monheim, Germany - Author
Univ Politecn Madrid, Dept Biotecnol, E-28040 Madrid, Spain - Author

Abstract

Fungi have an absolute requirement for K+, but K+ may be partially replaced by Na+. Na+ uptake in Ustilago maydis and Pichia sorbitophila was found to exhibit a fast rate, low K-m, and apparent independence of the membrane potential. Searches of sequences with similarity to P-type ATPases in databases allowed us to identify three genes in these species, Umacu1, Umacu2, and PsACU1, that could encode P-type ATPases of a novel type. Deletion of the acu1 and acu2 genes proved that they encoded the transporters that mediated the high-affinity Na+ uptake of U. maydis. Heterologous expressions of the Umacu2 gene in K+ transport mutants of Saccharomyces cerevisiae and transport studies in the single and double Deltaacu1 and Deltaacu2 mutants of U. maydis revealed that the acu1 and acu2 genes encode transporters that mediated high-affinity K+ uptake in addition to Na+ uptake. Other fungi also have genes or pseudogenes whose translated sequences show high similarity to the ACU proteins of U. maydis and P. sorbitophila. In the phylogenetic tree of P-type ATPases all the identified ACU ATPases define a new cluster, which shows the lowest divergence with type IIC, animal Na+,K+-ATPases. The fungal high-affinity Na+ uptake mediated by ACU ATPases is functionally identical to the uptake that is mediated by some plant HKT transporters.

Keywords

Adenosine triphosphatasesAmino acid sequenceCation-transportEnergy transductionEscherichia-coliFungal proteinsFungiGastric h,k-atpaseIon transportMolecular sequence dataMolecular-cloningNeurospora-crassaPhylogenyPichiaPlasma-membrane atpasePotassiumSaccharomyces-cerevisiaeSchwanniomyces-occidentalisSequence alignmentSequence deletionSodiumUstilagoUstilago-maydis

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Eukaryotic Cell due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2004, it was in position 16/84, thus managing to position itself as a Q1 (Primer Cuartil), in the category Microbiology.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 3.75, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions Jul 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-07-08, the following number of citations:

  • WoS: 59
  • Scopus: 64
  • Europe PMC: 35

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-07-08:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 55.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 55 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 4.
  • The number of mentions on Wikipedia: 8 (Altmetric).

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Germany.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (GARCIA DE BLAS GONZALEZ, MARIA BLANCA) and Last Author (RODRIGUEZ NAVARRO, ALONSO).

the author responsible for correspondence tasks has been RODRIGUEZ NAVARRO, ALONSO.