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This work was supported by the Spanish Ministerio de Ciencia e Innovacion; the ERDF European program [grant No. AGL2007-61075, and a fellowship to S.A.M.]; the DGUI-UPM Research Group Program.

Analysis of institutional authors

Rodriguez-Navarro, AlonsoAuthorHaro, RosarioCorresponding Author

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Knockouts of Physcomitrella patens CHX1 and CHX2 Transporters Reveal High Complexity of Potassium Homeostasis

Publicated to:Plant And Cell Physiology. 54 (9): 1455-1468 - 2013-09-01 54(9), DOI: 10.1093/pcp/pct096

Authors: Mottaleb, Shady A.; Rodriguez-Navarro, Alonso; Haro, Rosario;

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Abstract

This study aims to increase our understanding of the functions of CHX transporters in plant cells using the model plant Physcomitrella patens, in which four CHX genes have been identified, PpCHX1-PpCHX4. Two of these genes, PpCHX1 and PpCHX2, are expressed at approximately the same level as the PpACT5 gene, but the other two genes show an extremely low expression. PpCHX1 and PpCHX2 restored growth of Escherichia coli mutants on low K+-containing media, suggesting that they mediated K+ uptake that may be energized by symport with H+. In contrast, these genes suppressed the defect associated with the kha1 mutation in Saccharomyces cerevisiae, which suggests that they might mediate K+/H+ antiport. PpCHX1-green fluorescent protein (GFP) fusion protein transiently expressed in P. patens protoplasts co-localized with a Golgi marker. In similar experiments, the PpCHX2-GFP protein appeared to localize to tonoplast and plasma membrane. We constructed the Delta Ppchx1 and Delta Ppchx2 single mutant lines, and the Delta Ppchx2 Delta Pphak1 double mutant. Single mutant plants grew normally under all the conditions tested and exhibited normal K+ and Rb+ influxes; the Delta Ppchx2 mutation did not increase the defect of Delta Pphak1 plants. In long-term experiments, Delta Ppchx2 plants showed slightly higher Rb+ retention than wild-type plants, which suggests that PpCHX2 mediates the transfer of Rb+ either from the vacuole to the cytosol or from the cytosol to the external medium in parallel with other transporters. The distinction between these two possibilities is technically difficult. We suggest that K+ transporters of several families are involved in the pH homeostasis of organelles by mediating either K+/H+ antiport or K+-H+ symport.

Keywords

chx transportersendomembranesAmino acid sequenceArabidopsisBryopsidaCation transport proteinsCell membraneChx transportersEndomembranesEscherichia-coliExpressionGene expression regulation, plantGene familyGene knockout techniquesGenetic complementation testGolgi apparatusGreen fluorescent proteinsHomeostasisIon transportK+ transportersKineticsMediates sodiumMembraneMolecular sequence dataMotive forceMutationPhylogenyPlant proteinsPotassiumPotassium fluxesPotassium-hydrogen antiportersProton electrochemical gradientProtoplastsReverse transcriptase polymerase chain reactionRolesRubidiumSaccharomyces cerevisiaeSaccharomyces cerevisiae proteinsSequence homology, amino acid

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Plant And Cell Physiology due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2013, it was in position 15/199, thus managing to position itself as a Q1 (Primer Cuartil), in the category Plant Sciences.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 2.32, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions Jun 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-06-07, the following number of citations:

  • WoS: 18
  • Scopus: 27
  • OpenCitations: 23

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-06-07:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 24.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 24 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 1.
  • The number of mentions on the social network X (formerly Twitter): 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Egypt.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Mottaleb, Shady A.) and Last Author (HARO HIDALGO, ROSARIO).

the author responsible for correspondence tasks has been HARO HIDALGO, ROSARIO.