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Analysis of institutional authors

Prieto, R. I.Corresponding AuthorImperial, J.AuthorPalacios, J. M.Corresponding AuthorBrito, B.Author

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June 9, 2019
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Functional and Expression Analysis of the Metal-Inducible dmeRF System from Rhizobium leguminosarum bv. viciae

Publicated to: APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 79 (20): 6414-6422 - 2013-10-10 79(20), DOI: 10.1128/AEM.01954-13

Authors:

Rubio-Sanz, L; Prieto, RI; Imperial, J; Palacios, JM; Brito, B
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Affiliations

CSIC, Madrid, Spain - Author
Univ Politecn Madrid, CBGP, Madrid, Spain - Author
Univ Politecn Madrid, Escuela Tecn Super Ingn Agr, Dept Biotecnol, Madrid, Spain - Author
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Abstract

A gene encoding a homolog to the cation diffusion facilitator protein DmeF from Cupriavidus metallidurans has been identified in the genome of Rhizobium leguminosarum UPM791. The R. leguminosarum dmeF gene is located downstream of an open reading frame (designated dmeR) encoding a protein homologous to the nickel-and cobalt-responsive transcriptional regulator RcnR from Escherichia coli. Analysis of gene expression showed that the R. leguminosarum dmeRF genes are organized as a transcriptional unit whose expression is strongly induced by nickel and cobalt ions, likely by alleviating the repressor activity of DmeR on dmeRF transcription. An R. leguminosarum dmeRF mutant strain displayed increased sensitivity to Co(II) and Ni(II), whereas no alterations of its resistance to Cd(II), Cu(II), or Zn(II) were observed. A decrease of symbiotic performance was observed when pea plants inoculated with an R. leguminosarum dmeRF deletion mutant strain were grown in the presence of high concentrations of nickel and cobalt. The same mutant induced significantly lower activity levels of NiFe hydrogenase in microaerobic cultures. These results indicate that the R. leguminosarum DmeRF system is a metal-responsive efflux mechanism acting as a key element for metal homeostasis in R. leguminosarum under free-living and symbiotic conditions. The presence of similar dmeRF gene clusters in other Rhizobiaceae suggests that the dmeRF system is a conserved mechanism for metal tolerance in legume endosymbiotic bacteria.
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Keywords

AcidCobaltEscherichia-coli rcnrGene deletionGene expression profilingHomeostasisHydrogenase activityLegume nodulesMembrane transport proteinsMetalliduransMicrobial sensitivity testsNickelNickel resistanceOperonPisum sativumProteinsRhizobium leguminosarumSymbiosisTranscription factorsZinc

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal APPLIED AND ENVIRONMENTAL MICROBIOLOGY due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2013, it was in position 24/119, thus managing to position itself as a Q1 (Primer Cuartil), in the category Microbiology.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations from Scopus Elsevier, it yields a value for the Field-Weighted Citation Impact from the Scopus agency: 1.1, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 13, 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2026-04-27, the following number of citations:

  • WoS: 31
  • Scopus: 35
  • Europe PMC: 19
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-27:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 40 (PlumX).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Additionally, the work has been submitted to a journal classified as Diamond in relation to this type of editorial policy.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://oa.upm.es/21407/

As a result of the publication of the work in the institutional repository, statistical usage data has been obtained that reflects its impact. In terms of dissemination, we can state that, as of

  • Views: 289
  • Downloads: 301
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Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Rubio-Sanz, L.) and Last Author (BRITO LOPEZ, MARIA BELEN).

the authors responsible for correspondence tasks have been Prieto Carbajo, Rosa Isabel and PALACIOS ALBERTI, JOSE MANUEL.

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Awards linked to the item

This work was supported by projects from Spain's MICINN (BIO2010-15301), Comunidad Autonoma de Madrid (S-505/AMB/0321 MICROAMBIENTE-CM), and Universidad Politecnica de Madrid [AL09-P(I+D)-06].
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