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Analysis of institutional authors

Navarro-Rodriguez, MonicaAuthorRubio, Luis M.Author

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June 9, 2019
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Role of Azotobacter vinelandii FdxN in FeMo-co biosynthesis

Publicated to: FEBS LETTERS. 588 (3): 512-516 - 2014-01-31 588(3), DOI: 10.1016/j.febslet.2013.12.018

Authors:

Jiménez-Vicente, E; Navarro-Rodríguez, M; Poza-Carrión, C; Rubio, LM
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Affiliations

Univ Politecn Madrid, Ctr Biotecnol & Genom Plantas - Author

Abstract

Biosynthesis of metal clusters for the nitrogenase component proteins NifH and NifDK involves electron donation events. Yet, electron donors specific to the biosynthetic pathways of the [ 4Fe-4S] cluster of NifH, or the P-cluster and the FeMo-co of NifDK, have not been identified. Here we show that an Azotobacter vinelandii mutant lacking fdxN was specifically impaired in FeMo-co biosynthesis. The Delta fdxN mutant produced 5-fold less NifB-co, an early FeMo-co biosynthetic intermediate, than wild type. As a consequence, it accumulated FeMo-co-deficient apo-NifDK and was impaired in NifDK activity. We conclude that FdxN plays a role in FeMo-co biosynthesis, presumably by donating electrons to support NifB-co synthesis by NifB. This is the first role in nitrogenase biosynthesis unequivocally assigned to any A. vinelandii ferredoxin. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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Keywords

eprferredoxiniron-molybdenum cofactornifbAzotobacter vinelandiiBinding siteBiosynthetic pathwaysElectronsEprFerredoxinFerredoxin-iGene-clusterIn-vitro synthesisIron compoundsIron-molybdenum cofactorMofe proteinMolybdoferredoxinMutationMutational analysisNifbNifb cofactorNitrogenaseNitrogenase componentsOxidoreductasesScaffold protein

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal FEBS LETTERS due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2014, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Biophysics. Notably, the journal is positioned above the 90th percentile.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2026-04-02:

  • WoS: 13
  • Scopus: 12
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-02:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 35.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 35 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 3.
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Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Jimenez-Vicente, Emilio) and Last Author (RUBIO HERRERO, LUIS MANUEL).

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Awards linked to the item

This work was supported by ERC Starting Grant 205442 and MICINN BIO2009-12661 Grant to LMR. We thank Luisa Maia and Pablo Gonzalez at the Chemistry Department of Universidade Nova de Lisboa for help using the EPR instrument. We thank Emma Barahona for the statistical analysis of Table 1 data.
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