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We gratefully acknowledge Pilar Paredes, Tomas Cascon and Pedro Mario Rodriguez-Pascual for excellent technical assistance, the Proteomic Service (ProteoRed, Centro Nacional de Biotecnologia) for MALDI-TOF/MS analysis, the gas chromatography service at Centro de Investigaciones Biologicas, Consejo Superior de Investigaciones Cientificas, for sugar determination and the infrared spectroscopy service at the Instituto de Ciencia de Materiales de Madrid, Consejo Superior de Investigaciones Cientificas for FTIR analysis. We thank Paul E. Staswick for providing SIJAR1 cDNA and F. Vangijsegem for providing the D. dadantii 3937 pel mutant strain. This work was supported by grants from the Spanish Ministerio de Ciencia e Innovacion (BIO2008-03052 to J.J.S.S., BIO2009-10784 to E.R., AGL-2009-12757 to E.L.S., BIO2008-04160 to S.P. and AGL2010-22300 to P.G.A.).

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Abelenda, Jose A.AuthorLopez-Solanilla, EmiliaAuthor

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Article

Jasmonate-dependent modifications of the pectin matrix during potato development function as a defense mechanism targeted by Dickeya dadantii virulence factors

Publicated to:Plant Journal. 77 (3): 418-429 - 2014-02-01 77(3), DOI: 10.1111/tpj.12393

Authors: Taurino, Marco; Abelenda, Jose A.; Rio-Alvarez, Isabel; Navarro, Cristina; Vicedo, Begonya; Farmaki, Theodora; Jimenez, Pedro; Garcia-Agustin, Pilar; Lopez-Solanilla, Emilia; Prat, Salome; Rojo, Enrique; Sanchez-Serrano, Jose J.; Sanmartin, Maite;

Affiliations

CSIC, Ctr Nacl Biotecnol, Dept Genet Mol Plantas, Madrid 28049, Spain - Author
Univ Jaume 1, Dept Ciencias Agr & Medio Nat, Lab Bioquim & Biotecnol, Area Fisiol Vegetal,Escuela Super Tecnol & Cienci, Castellon de La Plana 12071, Spain - Author
Univ Politecn Madrid, Ctr Biotecnol & Genom Plantas, Escuela Tecn Super Ingenieros Agron, Madrid 28223, Spain - Author

Abstract

The plant cell wall constitutes an essential protection barrier against pathogen attack. In addition, cell-wall disruption leads to accumulation of jasmonates (JAs), which are key signaling molecules for activation of plant inducible defense responses. However, whether JAs in return modulate the cell-wall composition to reinforce this defensive barrier remains unknown. The enzyme 13-allene oxide synthase (13-AOS) catalyzes the first committed step towards biosynthesis of JAs. In potato (Solanum tuberosum), there are two putative St13-AOS genes, which we show here to be differentially induced upon wounding. We also determine that both genes complement an Arabidopsis aos null mutant, indicating that they encode functional 13-AOS enzymes. Indeed, transgenic potato plants lacking both St13-AOS genes (CoAOS1/2 lines) exhibited a significant reduction of JAs, a concomitant decrease in wound-responsive gene activation, and an increased severity of soft rot disease symptoms caused by Dickeya dadantii. Intriguingly, a hypovirulent D.dadantii pel strain lacking the five major pectate lyases, which causes limited tissue maceration on wild-type plants, regained infectivity in CoAOS1/2 plants. In line with this, we found differences in pectin methyl esterase activity and cell-wall pectin composition between wild-type and CoAOS1/2 plants. Importantly, wild-type plants had pectins with a lower degree of methyl esterification, which are the substrates of the pectate lyases mutated in the pel strain. These results suggest that, during development of potato plants, JAs mediate modification of the pectin matrix to form a defensive barrier that is counteracted by pectinolytic virulence factors from D.dadantii.

Keywords

allene oxide synthasecell walldickeyajasmonic acidopdaAcidAllene oxide synthaseArabidopsis-thalianaCell wallDickeyaErwinia-chrysanthemiFt-irJasmonic acidMolecular-cloningOctadecanoid biosynthesisOpdaPectin methyl-esterificationProteinase-inhibitorsSignal-transductionWound response

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Plant Journal due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2014, it was in position 10/204, thus managing to position itself as a Q1 (Primer Cuartil), in the category Plant Sciences.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 2.45, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions Jun 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-06-16, the following number of citations:

  • WoS: 18
  • Scopus: 25
  • OpenCitations: 26

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-06-16:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 72.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 72 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 0.5.
  • The number of mentions on the social network X (formerly Twitter): 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Greece.