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June 17, 2019
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Article

Xyloglucan endotransglucosylase/hydrolase (XTH) overexpression affects growth and cell wall mechanics in etiolated Arabidopsis hypocotyls

Publicated to: JOURNAL OF EXPERIMENTAL BOTANY. 64 (8): 2481-2497 - 2013-05-01 64(8), DOI: 10.1093/jxb/ert107

Authors:

Miedes, E; Suslov, D; Vandenbussche, F; Kenobi, K; Ivakov, A; Van Der Straeten, D; Lorences, EP; Mellerowicz, EJ; Verbelen, JP; Vissenberg, K
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Affiliations

Interacciones Moleculares Planta-Patógeno. Universidad Politécnica de Madrid - Author
Max Planck Inst Mol Plant Physiol - Author
Max Planck Institute of Molecular Plant Physiology - Author
Saint Petersburg State University - Author
St Petersburg State Univ, Fac Biol & Soil Sci, Dept Plant Physiol & Biochem - Author
Sveriges lantbruksuniversitet - Author
Umea Plant Sci Ctr, SLU, Dept Forest Genet & Plant Physiol - Author
Univ Antwerp, Dept Biol Plant Growth & Dev - Author
Univ Ghent, Lab Funct Plant Biol, Dept Physiol - Author
Univ Nottingham, Ctr Plant Integrat Biol - Author
Univ Valencia, Fac Farm, Dept Biol Vegetal, E-46100 Burjassot - Author
Universidad Politécnica de Madrid - Author
Universiteit Antwerpen - Author
Universiteit Gent - Author
University of Nottingham - Author
University of Valencia - Author
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Abstract

Growth and biomechanics of etiolated hypocotyls from Arabidopsis thaliana lines overexpressing xyloglucan endotransglucosylase/hydrolase AtXTH18, AtXTH19, AtXTH20, and PttXET16-34 were studied. Overexpression of AtXTH18, AtXTH19, and AtXTH20 stimulated growth of hypocotyls, while PttXET16-34 overexpression did not show this effect. In vitro extension of frozen/thawed hypocotyls measured by a constant-load extensiometer started from a high-amplitude initial deformation followed by a slow time-dependent creep. Creep of growing XTH-overexpressing (OE) hypocotyls was more linear in time compared with the wild type at pH 5.0, reflecting their higher potential for long-term extension. XTH-OE plants deposited 65-84% more cell wall material per hypocotyl cross-sectional area than wild-type plants. As a result, their wall stress under each external load was lower than in the wild-type. Growing XTH-OE hypocotyls had higher values of initial deformation stress-1 compared with the wild type. Plotting creep rates for each line under different loads against the respective wall stress values gave straight lines. Their slopes and intercepts with the abscissa correspond to φ (in vitro cell wall extensibility) and y (in vitro cell wall yield threshold) values characterizing cell wall material properties. The wall material in XTH-OE lines was more pliant than in the wild type due to lower y values. In contrast, the acid-induced wall extension in vitro resulted from increasing φ values. Thus, three factors contributed to the XTH-OE-stimulated growth in Arabidopsis hypocotyls: their more linear creep, higher values of initial deformation-stress-1, and lower y values. © 2013 © The Author(2) [2013].
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Keywords

arabidopsis hypocotylcell wallcreep testextensiometryxthArabidopsis hypocotylCell wallCreep testExtensiometryGrowthXth

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal JOURNAL OF EXPERIMENTAL BOTANY due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2013, it was in position 11/199, thus managing to position itself as a Q1 (Primer Cuartil), in the category Plant Sciences.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations provided by WoS (ESI, Clarivate), it yields a value for the citation normalization relative to the expected citation rate of: 2.92. This indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 13, 2025)

This information is reinforced by other indicators of the same type, which, although dynamic over time and dependent on the set of average global citations at the time of their calculation, consistently position the work at some point among the top 50% most cited in its field:

  • Weighted Average of Normalized Impact by the Scopus agency: 3.88 (source consulted: FECYT Mar 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2026-04-27, the following number of citations:

  • WoS: 106
  • Scopus: 127
  • Europe PMC: 51
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-27:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 136.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 135 (PlumX).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://oa.upm.es/29012/

As a result of the publication of the work in the institutional repository, statistical usage data has been obtained that reflects its impact. In terms of dissemination, we can state that, as of

  • Views: 389
  • Downloads: 553
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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Belgium; Germany; Russia; Sweden; United Kingdom.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (MIEDES VICENTE, EVA) .

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