July 27, 2019
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Article

The Pseudomonas syringae pv. tomato DC3000 PSPTO_0820 multidrug transporter is involved in resistance to plant antimicrobials and bacterial survival during tomato plant infection

Publicated to: PLoS One. 14 (6): e0218815- - 2019-06-25 14(6), DOI: 10.1371/journal.pone.0218815

Authors:

Santamaria-Hernando, Saray; Senovilla, Marta; Gonzalez-Mula, Almudena; Manuel Martinez-Garcia, Pedro; Nebreda, Sandra; Rodriguez-Palenzuela, Pablo; Lopez-Solanilla, Emilia; Juan Rodriguez-Herva, Jose
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Affiliations

Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria - Author
Interacciones Moleculares Planta-Patógeno. Universidad Politécnica de Madrid - Author
Univ Pablo Olavide, Univ Sevilla, CSIC, Ctr Andaluz Biol Mol & Med Regenerat CABIMER - Author
Univ Politecn Madrid, Escuela Tecn Super Ingn Agron Alimentaria & Biosi, Dept Biotecnol Biol Vegetal - Author
Univ Politecn Madrid, Inst Nacl Invest & Tecnol Agr & Alimentaria, CBGP - Author
Universidad Autónoma de Madrid - Author
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Abstract

Multidrug resistance efflux pumps protect bacterial cells against a wide spectrum of antimicrobial compounds. PSPTO_0820 is a predicted multidrug transporter from the phytopathogenic bacterium Pseudomonas syringae pv. tomato DC3000. Orthologs of this protein are conserved within many Pseudomonas species that interact with plants. To study the potential role of PSPTO_0820 in plant-bacteria interaction, a mutant in this gene was isolated and characterized. In addition, with the aim to find the outer membrane channel for this efflux system, a mutant in PSPTO_4977, a TolC-like gene, was also analyzed. Both mutants were more susceptible to trans-cinnamic and chlorogenic acids and to the flavonoid (+)-catechin, when added to the culture medium. The expression level of both genes increased in the presence of (+)-catechin and, in the case of PSPTO_0820, also in response to trans-cinnamic acid. PSPTO_0820 and PSPTO_4977 mutants were unable to colonize tomato at high population levels. This work evidences the involvement of these two proteins in the resistance to plant antimicrobials, supporting also the importance of chlorogenic acid, trans-cinnamic acid, and (+)-catechin in the tomato plant defense response against P. syringae pv. tomato DC3000 infection.
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Keywords

Anti-infective agentsAntimicrobial cationic peptidesAtp binding cassette transporter, subfamily bBacterial proteinsGenes, bacterialHost microbial interactionsMutationPlant diseasesPlant proteinsPseudomonas syringaeSolanum lycopersicumVirulence

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2026-04-27:

  • WoS: 14
  • Scopus: 16
  • Europe PMC: 3
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-27:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 59.

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 2.
  • The number of mentions on the social network X (formerly Twitter): 2 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://oa.upm.es/86226/

As a result of the publication of the work in the institutional repository, statistical usage data has been obtained that reflects its impact. In terms of dissemination, we can state that, as of

  • Views: 158
  • Downloads: 24
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Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (SANTAMARIA HERNANDO, SARAY) and Last Author (RODRIGUEZ HERVA, JOSE JUAN).

the author responsible for correspondence tasks has been RODRIGUEZ HERVA, JOSE JUAN.

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