February 4, 2014
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Article

Graph Based Study of Allergen Cross-Reactivity of Plant Lipid Transfer Proteins (LTPs) Using Microarray in a Multicenter Study

Publicated to: PLoS One. 7 (12): e50799- - 2012-12-14 7(12), DOI: 10.1371/journal.pone.0050799

Authors:

Palacín, A; Gómez-Casado, C; Rivas, LA; Aguirre, J; Tordesillas, L; Bartra, J; Blanco, C; Carrillo, T; Cuesta-Herranz, J; de Frutos, C; Alvarez-Eire, GG; Fernández, FJ; Gamboa, P; Muñoz, R; Sánchez-Monge, R; Sirvent, S; Torres, MJ; Varela-Losada, S; Rodríguez, R; Parro, V; Blanca, M; Salcedo, G; Díaz-Perales, A
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Affiliations

Centro de Biotecnologia y Genomica de Plantas, Universidad Politecnica de Madrid - Author
Complexo Hosp, Unidad Alergol - Author
Ctr Astrobiol INTA CSIC, Dept Evoluc Mol - Author
Ctr Biotecnol & Genom Plantas UPM INIA - Author
Fdn Jimenez Diaz, Unidad Serv Alergia - Author
Hosp Basurto, Serv Alergia - Author
Hosp Civil, Carlos Haya Hosp, Fdn IMABIS, Res Lab - Author
Hosp Univ Gran Canaria Dr Negrin, Serv Alergol - Author
Hosp Univ Princesa, Inst Invest Sanitaria Princesa IP, Serv Alergia - Author
Miguel Hernandez Univ, Gen Univ Hosp, Allergy Sect - Author
Univ Barcelona, Unitat Alergia Serv Pneumol & Alergia Resp, Inst Invest Biomed August Pi i Sunyer IDIBAPS, Ctr Invest Biomed Red Enfermedad Resp CIBERS - Author
Univ Complutense, Fac Quim, Dept Bioquim & Biol Mol 1 - Author
Univ Politecn Madrid, ETSI Agronomos, Dept Biotecnol - Author
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Abstract

The study of cross-reactivity in allergy is key to both understanding. the allergic response of many patients and providing them with a rational treatment In the present study, protein microarrays and a co-sensitization graph approach were used in conjunction with an allergen microarray immunoassay. This enabled us to include a wide number of proteins and a large number of patients, and to study sensitization profiles among members of the LTP family. Fourteen LTPs from the most frequent plant food-induced allergies in the geographical area studied were printed into a microarray specifically designed for this research. 212 patients with fruit allergy and 117 food-tolerant pollen allergic subjects were recruited from seven regions of Spain with different pollen profiles, and their sera were tested with allergen microarray. This approach has proven itself to be a good tool to study cross-reactivity between members of LTP family, and could become a useful strategy to analyze other families of allergens.
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Keywords

BindingCloningDomainsElisaExpressionImmunizationLepidopteraLinked-immunosorbent-assayPollenPredation

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal PLoS One due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2012, it was in position 7/56, thus managing to position itself as a Q1 (Primer Cuartil), in the category Multidisciplinary Sciences.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations provided by WoS (ESI, Clarivate), it yields a value for the citation normalization relative to the expected citation rate of: 1.2. This indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 13, 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2026-04-27, the following number of citations:

  • WoS: 57
  • Scopus: 63
  • Europe PMC: 20
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-27:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 76.

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 4.
  • The number of mentions on the social network Facebook: 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://oa.upm.es/16187/

As a result of the publication of the work in the institutional repository, statistical usage data has been obtained that reflects its impact. In terms of dissemination, we can state that, as of

  • Views: 478
  • Downloads: 294
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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: San Francisco.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Palacin, A) and Last Author (Diaz-Perales, A).

the author responsible for correspondence tasks has been Diaz-Perales, A.

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