Context Romania is currently one of the best-connected landscapes, with high-quality habitats and a high density of brown bears. However, regional development measures are needed for the nation's economic growth, and without responsible government actions, these measures pose a threat to brown bear connectivity, a vital pillar of the entire Carpathian region. Despite having important implications for the species conservation, bears sex-specific behaviour has been overlooked in connectivity assessments. Objectives The study's main goals were to (i) develop sex-specific distribution and connectivity models, (ii) assess the movement patterns differences between females and males, and (iii) identify high-quality areas to be secured for species connectivity conservation. Methods We designed a methodological framework based on interdisciplinary approaches and modelling tools for landscape connectivity combined with novel spatial analysis. Telemetry data of sixteen bears (females and males) were utilized to predict habitat selection (MaxEnt), and circuit-theoretic approaches (Circuitscape) were applied to identify the potential movement corridors of both sexes and high-priority areas. Results The obtained results provided new evidence on the uneven movement patterns and pathways used by males and females in the study region. Males' movement preferences were related to river shores and dense forest coverage areas, avoiding urban areas, while females were predicted to move across forested and open areas and were more tolerant to human-modified areas. Three high-quality areas were delineated for both sexes, and connectivity conservation efforts are needed due to the vicinity of anthropic pressures. Conclusions This study depicts the usefulness of combining interdisciplinary approaches and provides planners, managers, and decision-makers with practical solutions to address sustainable design and management within the intervened landscapes. Using separate female and male spatial models for leading management and conservation measures, setting connectivity targets within environmental and impact assessments while adopting conservation easements could secure a safety network for biodiversity conservation in the Carpathians.