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Analysis of institutional authors

Herreros P.AuthorTapia-González S.AuthorHeras M.f.l.AuthorHolgado M.Corresponding Author

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March 7, 2022
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Alternative Brain Slice-on-a-Chip for Organotypic Culture and Effective Fluorescence Injection Testing

Publicated to:International Journal Of Molecular Sciences. 23 (5): 2549- - 2022-03-01 23(5), DOI: 10.3390/ijms23052549

Authors: Herreros, Pedro; Tapia-Gonzalez, Silvia; Sanchez-Olivares, Laura; Heras, Maria Fe Laguna; Holgado, Miguel

Affiliations

CIBER Enfermedades Neurodegenerativas , Universidad Politécnica de Madrid , Consejo Superior de Investigaciones Científicas - Author
CSIC, Inst Cajal, Dept Neurobiol Func & Sistemas, Madrid 28002, Spain - Author
Hosp Clin San Carlos, Hlth Res Inst, Grp Organ & Tissue On A Chip & Invitro Detect, Madrid 28040, Spain - Author
Hospital Clínico San Carlos de Madrid , Universidad Politécnica de Madrid - Author
ISCIII, Ctr Invest Biomed Red Enfermedades Neurodegenerat, Madrid 28031, Spain - Author
Univ Politecn Madrid, Dept Appl Phys & Mat Engn, Escuela Tecn Super Ingn Ind, Madrid 28006, Spain - Author
Univ Politecn Madrid, Grp Opt Photon & Biophoton GOFB, Ctr Biomed Technol, Pozuelo De Alarcon 28223, Spain - Author
Univ Politecn Madrid, Lab Cajal Circuitos Cort CTB, Madrid 28223, Spain - Author
Universidad Politécnica de Madrid - Author
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Abstract

Mouse brain slices are one of the most common models to study brain development and functioning, increasing the number of study models that integrate microfluidic systems for hippocampal slice cultures. This report presents an alternative brain slice-on-a-chip, integrating an injection system inside the chip to dispense a fluorescent dye for long-term monitoring. Hippocampal slices have been cultured inside these chips, observing fluorescence signals from living cells, maintaining the cytoarchitecture of the slices. Having fluorescence images of biological samples inside the chip demonstrates the effectiveness of the staining process using the injection method avoiding leaks or biological contamination. The technology developed in this study presents a significant improvement in the local administration of reagents within a brain slice-on-a-chip system, which could be a suitable option for organotypic cultures in a microfluidic chip acting as a highly effective bioreactor.

Keywords

brain slicecell labellingfluorescence imagingorgan-on-a-chipperfusionAnimalsBrainBrain sliceCell labellingFluorescence imagingHippocampusLab-on-a-chip devicesMiceMicrofluidicsNeurogenesisOrgan culture techniquesOrgan-on-a-chip

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal International Journal Of Molecular Sciences due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2022, it was in position 66/285, thus managing to position itself as a Q1 (Primer Cuartil), in the category Biochemistry & Molecular Biology.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 2.42, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions Jul 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-07-07, the following number of citations:

  • WoS: 8
  • Scopus: 8

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-07-07:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 33.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 33 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 4.45.
  • The number of mentions on the social network X (formerly Twitter): 7 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (HERREROS PEREZ, PEDRO) and Last Author (HOLGADO BOLAÑOS, MIGUEL).

the author responsible for correspondence tasks has been HOLGADO BOLAÑOS, MIGUEL.