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Analysis of institutional authors

Saez CAuthorSaez, CristinaAuthor

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September 15, 2024
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Genetic Dissection of ToLCNDV Resistance in Resistant Sources of Cucumis melo

Publicated to: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 25 (16): 8880- - 2024-08-01 25(16), DOI: 10.3390/ijms25168880

Authors:

Pérez-Moro, C; Sáez, C; Sifres, A; Lopez, C; Dhillon, NPS; Picó, B; Pérez-de-Castro, A
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Affiliations

Kasetsart Univ, World Vegetable Ctr, Kamphaeng Saen 73140, Nakhon Pathom, Thailand - Author
Univ Politecn Madrid, Ctr Biotecnol & Genomica Plantas UPM INIA, Madrid 28031, Spain - Author
Univ Politecn Madrid, ETS Ingn Agron Alimentaria & Biosistemas, Madrid 28031, Spain - Author
Univ Politecn Valencia, Inst Conservac & Mejora Agrodivers Valenciana COMA, Camino Vera S-N, Valencia 46022, Spain - Author
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Abstract

Tomato leaf curl New Delhi virus (ToLCNDV) is a begomovirus causing significant melon (Cucumis melo) crop losses globally. This study aims to map the ToLCNDV resistance in the PI 414723 melon accession, previously identified and characterized through phenotypic studies, thereby exploring shared genomic regions with the established resistant source WM-7. In the present study, WM-7 and PI 414723 were crossed with the susceptible accessions 'Rochet' and 'Blanco' respectively, to generate F-1 hybrids. These hybrids were self-pollinated to generate the populations for mapping the ToLCNDV resistance region and designing markers for marker-assisted selection. Disease evaluation included visual symptom scoring, viral-load quantification and tissue printing. Genotyping-by-sequencing and SNP markers were used for quantitative trait loci (QTL) mapping. For genetic analysis, qPCR and bulked segregant RNA-seq (BSR-seq) were performed. Gene expression was assessed using RNA-seq, and qRT-PCR was used for confirmation. The research narrows the candidate region for resistance in WM-7 and identifies overlapping QTLs on chromosome 11 in PI 414723, found in the region of the DNA primase large subunit. BSR-seq and expression analyses highlight potential regulatory roles of chromosome 2 in conferring resistance. Differential expression was confirmed for six genes in the candidate region on chromosome 2. This study confirms the existence of common resistance genes in PI 414723 and WM-7.
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Keywords

AlignmentBegomovirusBsr-seqChromosome mappingCucumis meloDisease resistanceFormatGene expressioGene expressionGenomeInfectionInheritanceLeveMelonNew-delhi-virusPhenotypePlant diseasesPolymorphism, single nucleotideQtlQuantitative trait lociTolcndvVariantVirus resistanceYellow mosaic-virusZucchini

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 72/320, thus managing to position itself as a Q1 (Primer Cuartil), in the category Biochemistry & Molecular Biology.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2026-04-27:

  • WoS: 5
  • Scopus: 6
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-27:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 19.

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 6.
  • The number of mentions on the social network X (formerly Twitter): 8 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://oa.upm.es/89402/

As a result of the publication of the work in the institutional repository, statistical usage data has been obtained that reflects its impact. In terms of dissemination, we can state that, as of

  • Views: 160
  • Downloads: 25
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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Thailand.

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Awards linked to the item

This work was supported by the grant PID2021-125787OR-C33, funded by MICIU/AEI/10.13039/501100011033; the 'FEDER UE' PROMETEO/2021/072 project for excellence groups Conselleria d'Educacio, Cultura, Universitats i Ocupacio (Generalitat Valenciana, Spain); and project RTC-2017-6023-2, funded MICIU/AEI/10.13039/501100011033 and by FEDER 'A way of making Europe'. C.S. was the recipient of a 'Margarita Salas' grant (MS/60) from the Requalification Program for Excellence of the Universitat Politecnica de Valencia, Spanish Ministry of Universities and European Union-Next Generation EU. C.P.M. is a recipient of the predoctoral fellowship "Programa de Ayudas de Investigacion y Desarrollo (PAID-01-19)" from Universitat Politecnica de Valencia. Funding for open access charge: Universitat Politecnica de Valencia (PAID-12-23).
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