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Grant support

This research was funded by Project AGL2009-11778 from Spanish Ministry of Science and Innovation and by VIROPLANT, a project that received funding from the European Union's Horizon 2020 Research and Innovation Program (Grant No. 773567).

Analysis of institutional authors

Ruiz-Padilla, AnaAuthorDonaire, LiviaAuthorAyllon, Maria ACorresponding Author

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November 12, 2024
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Reexamining the Mycovirome of Botrytis spp.

Publicated to:Viruses-Basel. 16 (10): 1640- - 2024-10-01 16(10), DOI: 10.3390/v16101640

Authors: Muñoz-Suárez, H; Ruiz-Padilla, A; Donaire, L; Benito, EP; Ayllón, MA

Affiliations

CSIC, Ctr Edafol & Biol Aplicada Segura CEBAS, Dept Stress Biol & Plant Pathol, Murcia 30100, Spain - Author
Univ Politecn Madrid UPM, Escuela Tecn Super Ingn Agron Alimentaria & Biosis, Dept Biotecnol Biol Vegetal, Madrid 28040, Spain - Author
Univ Politecn Madrid, Ctr Biotecnol & Genom Plantas, Inst Nacl Invest & Tecnol Agr & Alimentaria UPM IN, CSIC, Madrid 28223, Spain - Author
Univ Salamanca, Dept Microbiol & Genet, Inst Invest Agrobiotecnol CIALE, C Rio Duero 12, Salamanca 37185, Spain - Author

Abstract

Botrytis species cause gray mold disease in more than 200 crops worldwide. To control this disease, chemical fungicides are usually applied. However, more sustainable control alternatives should be explored, such as the use of hypovirulent mycovirus-infected fungal strains. To determine the mycovirome of two Botrytis species, B. cinerea and B. prunorum, we reanalyzed RNA-Seq and small RNA-Seq data using different assembly programs and an updated viral database, aiming to identify new mycoviruses that were previously not described in the same dataset. New mycoviruses were identified, including those previously reported to infect or be associated with B. cinerea and Plasmopara viticola, such as Botrytis cinerea alpha-like virus 1 and Plasmopara viticola lesion-associated ourmia-like virus 80. Additionally, two novel narnaviruses, not previously identified infecting Botrytis species, have been characterized, tentatively named Botrytis cinerea narnavirus 1 and Botrytis narnavirus 1. The analysis of small RNAs suggested that all identified mycoviruses were targeted by the antiviral fungal mechanism, regardless of the viral genome type. In conclusion, the enlarged list of newly found viruses and the application of different bioinformatics approaches have enabled the identification of novel mycoviruses not previously described in Botrytis species, expanding the already extensive list.

Keywords

<italic>botrytis cinerea</italic><italic>botrytis prunorum</italic><italic>narnaviridae</italic>AlignmentAmino acid sequenceAmino acid substitutionAntiviral mechanismArabidopsisArabidopsis thalianaArticleBioinformaticsBluetongue orbivirusBotrytisBotrytis cinereaBotrytis prunorumBotrytis cinereaChestnut blightCinereaClassificationDna extractionDouble-stranded-rnaEvolutionFungal virusesFungusGeneticsGenome, viralHeterobasidionHigh throughput sequencinHigh throughput sequencingHypovirulencIsolation and purificationMetagenomicsMicrobiologyMyceliumMycoviromeMycovirusMycovirusesNarnaviridaeNonhumanPhylogenetic treePhylogenyPlantPlant diseasePlant diseasesPlant immunityPlant virusReconstructionRna extractionRna sequenceRna silencingRna, viralSclerotinia sclerotiorumVirologyViromeVirusVirus genomeVirus rnaWhole genome sequencing

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Viruses-Basel due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Infectious Diseases.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-08-25:

  • Scopus: 1

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-08-25:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 2 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

    It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

    • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
    • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://oa.upm.es/89302/

    As a result of the publication of the work in the institutional repository, statistical usage data has been obtained that reflects its impact. In terms of dissemination, we can state that, as of

    • Views: 37
    • Downloads: 6

    Leadership analysis of institutional authors

    There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Munoz-Suarez, Hugo) and Last Author (AYLLON TALAVERA, MARIA ANGELES).

    the author responsible for correspondence tasks has been AYLLON TALAVERA, MARIA ANGELES.