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This research was funded by Comunidad de Madrid within the framework of the Convenio Plurianual con la Universidad Politecnica de Madrid en la linea de actuacion Programa de Excelencia para el Profesorado Universitario (E.M., M190020074BEMV). We also acknowledge financial support from the Universidad Politecnica de Madrid through the projects VAGI23JPMC and VAGI24JPMC. C.K. was funded by the Algerian government (057Bis/PG/Espagne/2020-2021). J.G. and J.M.P. were funded by the Spanish Ministry of Science and Innovation (MICINN) through the ChaSisCOMA (PID2021-122711NB-C21) project. K.V. was supported by the University of Antwerp (BOF-NOI) and the Research Foundation Flanders (FWO, grant G013023N).
Analysis of institutional authors
Khoulali, CeliaAuthorPastor, Juan ManuelAuthorGaleano, JavierAuthorMiedes, EvaCorresponding AuthorCell Wall-Based Machine Learning Models to Predict Plant Growth Using Onion Epidermis
Publicated to:International Journal Of Molecular Sciences. 26 (7): 2946- - 2025-03-24 26(7), DOI: https://doi.org/10.3390/ijms26072946
Authors: Khoulali, Celia; Pastor, Juan Manuel; Galeano, Javier; Vissenberg, Kris; Miedes, Eva
Affiliations
Abstract
The plant cell wall (CW) is a physical barrier that plays a dual role in plant physiology, providing structural support for growth and development. Understanding the dynamics of CW growth is crucial for optimizing crop yields. In this study, we employed onion (Allium cepa L.) epidermis as a model system, leveraging its layered organization to investigate growth stages. Microscopic analysis revealed proportional variations in cell size in different epidermal layers, offering insights into growth dynamics and CW structural adaptations. Fourier transform infrared spectroscopy (FTIR) identified 11 distinct spectral intervals associated with CW components, highlighting structural modifications that influence wall elasticity and rigidity. Biochemical assays across developmental layers demonstrated variations in cellulose, soluble sugars, and antioxidant content, reflecting biochemical shifts during growth. The differential expression of ten cell wall enzyme (CWE) genes, analyzed via RT-qPCR, revealed significant correlations between gene expression patterns and CW composition changes across developmental layers. Notably, the gene expression levels of the pectin methylesterase and fucosidase enzymes were associated with the contents in cellulose, soluble sugar, and antioxidants. To complement these findings, machine learning models, including Support Vector Machines (SVM), k-Nearest Neighbors (kNN), and Neural Networks, were employed to integrate FTIR data, biochemical parameters, and CWE gene expression profiles. Our models achieved high accuracy in predicting growth stages. This underscores the intricate interplay among CW composition, CW enzymatic activity, and growth dynamics, providing a predictive framework with applications in enhancing crop productivity and sustainability.
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Bibliometric impact. Analysis of the contribution and dissemination channel
The work has been published in the journal International Journal Of Molecular Sciences due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2025, it was in position 68/231, thus managing to position itself as a Q1 (Primer Cuartil), in the category Chemistry, Multidisciplinary.
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Leadership analysis of institutional authors
This work has been carried out with international collaboration, specifically with researchers from: Belgium; Greece.
There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (KHOULALI, CELIA) and Last Author (MIEDES VICENTE, EVA).
the author responsible for correspondence tasks has been MIEDES VICENTE, EVA.