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Analysis of institutional authors

Mugica-Galan, PaulaAuthorMiro-Bueno, JesusAuthorHueso-Gil, AngelesAuthorJapon, PabloAuthorGoni-Moreno, AngelCorresponding Author

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July 31, 2025
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Standardized Quorum Sensing Tools for Gram-Negative Bacteria

Publicated to: Acs Synthetic Biology. 14 (6): 2380-2385 - 2025-06-06 14(6), DOI: 10.1021/acssynbio.5c00036

Authors:

Mugica-Galan, Paula; Miro-Bueno, Jesus; Hueso-Gil, Angeles; Japon, Pablo; Goni-Moreno, Angel
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Affiliations

CSIC, Ctr Nacl Biotecnol, Syst Biol Dept, Madrid 28049, Spain - Author
Univ Politecn Madrid, Madrid 28223, Spain - Author

Abstract

Engineering synthetic consortia to perform distributed functions requires robust quorum sensing (QS) systems to facilitate communication between cells. However, the current QS toolbox lacks standardized implementations, which are particularly valuable for use in bacteria beyond the model species Escherichia coli. We developed a set of three QS systems encompassing both sender and receiver modules, constructed using backbones from the SEVA (Standard European Vector Architecture) plasmid collection. This increases versatility, allowing plasmid features like the origin of replication or antibiotic marker to be easily swapped. The systems were characterized using the synthetic biology chassis Pseudomonas putida. We first tested individual modules, then combined sender and receiver modules in the same host, and finally assessed the performance across separate cells to evaluate consortia dynamics. Alongside the QS set, we provide mathematical models and rate parameters to support the design efforts. Together, these tools advance the engineering of robust and predictable multicellular functions.
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Keywords

BacteriBacteriaCell-cell communicationCell−cell communicationDistributed computationGenetic toolsProteiQuorum sensingStandardsSystem

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Acs Synthetic Biology due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2025, it was in position 18/86, thus managing to position itself as a Q1 (Primer Cuartil), in the category Biochemical Research Methods.

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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-12-22:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 4.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 6 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 8.
  • The number of mentions on the social network X (formerly Twitter): 1 (Altmetric).
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Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (MUGICA GALAN, PAULA) and Last Author (GOÑI MORENO, ANGEL).

the author responsible for correspondence tasks has been GOÑI MORENO, ANGEL.

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Awards linked to the item

This work was supported by the ECCO (ERC-2021-COG-101044360) Contract of the EU, and grants MULTI-SYSBIO (PID2020-117205GA-I00), BIOELECTRIC (CNS2022-135951), and MULTISYNBIO (PID2023-152470NB-I00) funded by MICIU/AEI/10.13039/501100011033.
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