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Analysis of institutional authors

Crevillen, PedroAuthorLopez, Juan A.AuthorJurado, SilviaAuthorPineiro, ManuelAuthorJarillo, Jose A.Corresponding Author

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April 9, 2019
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Article

Arabidopsis SWC4 Binds DNA and Recruits the SWR1 Complex to Modulate Histone H2A.Z Deposition at Key Regulatory Genes

Publicated to: Molecular Plant. 11 (6): 815-832 - 2018-06-04 11(6), DOI: 10.1016/j.molp.2018.03.014

Authors:

Gómez-Zambrano, A; Crevillén, P; Franco-Zorrilla, JM; López, JA; Moreno-Romero, J; Roszak, P; Santos-González, J; Jurado, S; Vázquez, J; Köhler, C; Solano, R; Piñeiro, M; Jarillo, JA
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Affiliations

CSIC, Genom Unit, Ctr Nacl Biotecnol, E-28049 Madrid, Spain - Author
CSIC, Inst Bioquim Vegetal & Fotosintesis, Seville 41092, Spain - Author
CSIC, Plant Mol Genet Dept, Ctr Nacl Biotecnol, E-28049 Madrid, Spain - Author
Ctr Nacl Invest Cardiovasc Carlos III CNIC, Lab Cardiovasc Prote, Madrid 28029, Spain - Author
Ctr Nacl Invest Cardiovasc Carlos III CNIC, Prote Unit, Madrid 28029, Spain - Author
Linnean Ctr Plant Biol, S-75652 Uppsala, Sweden - Author
Sainsbury Lab, Cambridge CB2 1LR, England - Author
Swedish Univ Agr Sci, Dept Plant Biol, Uppsala BioCtr, S-75652 Uppsala, Sweden - Author
UB, CRAG, CSIC, IRTA,UAB, Campus UAB, Barcelona, Spain - Author
Univ Politecn Madrid, Ctr Biotecnol & Genom Plantas, Inst Nacl Invest & Tecnol Agr & Alimentaria INIA, Campus Montegancedo UPM, Madrid 28223, Spain - Author
Univ Seville, Seville 41092, Spain - Author
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Abstract

Deposition of the H2A.Z histone variant by the SWR1 complex (SWR1-C) in regulatory regions of specific loci modulates transcription. Characterization of mutations in Arabidopsis thaliana homologs of yeast SWR1-C has revealed a role for H2A.Z exchange in a variety of developmental processes. Nevertheless, the exact composition of plant SWR1-C and how it is recruited to target genes remains to be established. Here we show that SWC4, the Arabidopsis homolog of yeast SANT domain protein Swc4/Eaf2, is a DNA-binding protein that interacts with SWR1-C subunits. We demonstrate that the swc4-1 knockout mutant is embryolethal, while SWC4 RNAi knockdown lines display pleiotropic phenotypic alterations in vegetative and reproductive traits, including acceleration of flowering time, indicating that SWC4 controls post-embryonic processes. Transcriptomic analyses and genome-wide profiling of H2A.Z indicate that SWC4 represses transcription of a number of genes, including the floral integrator FT and key transcription factors, mainly by modulating H2A.Z deposition. Interestingly, SWC4 silencing does not affect H2A.Z deposition at the FLClocus nor expression of this gene, a master regulator of flowering previously shown to be controlled by SWR1-C. Importantly, we find that SWC4 recognizes specific AT-rich DNA elements in the chromatin regions of target genes and that SWC4 silencing impairs SWR1-C binding at FT. Collectively, our data suggest that SWC4 regulates plant growth and development by aiding SWR1-C recruitment and modulating H2A.Z deposition.
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Keywords

activationchromatinfloral repressionflowering responsesflowering timeh2a.z depositionlocus-c expressionmodifying complexesnua4nuclear actinsant domainswc4swr1 complexvariant h2a.zArabidopsisArabidopsis proteinsChromatinChromatin-remodeling complexDna, plantDna-binding proteinsFlowering timeFlowersGc rich sequenceGene expression regulation, plantGene knockdown techniquesH2a.z depositionHistonesProtein bindingSeedsSwc4Swr1 complex

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Molecular Plant due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2018, it was in position 4/228, thus managing to position itself as a Q1 (Primer Cuartil), in the category Plant Sciences. Notably, the journal is positioned above the 90th percentile.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations provided by WoS (ESI, Clarivate), it yields a value for the citation normalization relative to the expected citation rate of: 1.79. This indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 13, 2025)

This information is reinforced by other indicators of the same type, which, although dynamic over time and dependent on the set of average global citations at the time of their calculation, consistently position the work at some point among the top 50% most cited in its field:

  • Weighted Average of Normalized Impact by the Scopus agency: 2.71 (source consulted: FECYT Mar 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2026-04-11, the following number of citations:

  • WoS: 61
  • Scopus: 60
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-11:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 113.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 113 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 12.
  • The number of mentions on the social network X (formerly Twitter): 20 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Sweden; United Kingdom.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Gomez-Zambrano, Angeles) and Last Author (JARILLO QUIROGA, JOSE ANTONIO).

the authors responsible for correspondence tasks have been Gomez-Zambrano, Angeles and JARILLO QUIROGA, JOSE ANTONIO.

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Awards linked to the item

This work was supported by grants BIO2010-15589, BIO2013-43098-R, and BIO2016-77559-R to J.A.J. and M.P., and grant RYC-2013-14689 to P.C. from the Spanish Ministerio de Economia y Competitividad (MINECO/FEDER, EU), and Marie Curie FP7-PEOPLE-2011-IEF grant 298790 to P.C. and J.A.J. from the European Commission. The CBGP is a Severo Ochoa Center of Excellence (SEV-2016-0672). The CNIC is supported by the Spanish Ministerio de Economia, Industria y Competitividad and the Pro CNIC Foundation, and is also a Severo Ochoa Center of Excellence (SEV-2015-0505).
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