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Analysis of institutional authors

Luengo-Oroz, Miguel A.AuthorPastor, DavidAuthorLedesma-Carbayo, MariaAuthorSantos, AndresAuthor

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June 9, 2019
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Methodology for Reconstructing Early Zebrafish Development From In Vivo Multiphoton Microscopy

Publicated to: IEEE TRANSACTIONS ON IMAGE PROCESSING. 21 (4): 2335-2340 - 2012-04-01 21(4), DOI: 10.1109/TIP.2011.2177911

Authors:

Luengo-Oroz, MA; Rubio-Guivernau, JL; Faure, E; Savy, T; Duloquin, L; Olivier, N; Pastor, D; Ledesma-Carbayo, M; Débarre, D; Bourgine, P; Beaurepaire, E; Peyriéras, N; Santos, A
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Affiliations

Biomat & Nanomed CIBER BBN, Ctr Invest Biomed Red Bioingn, Madrid, Spain - Author
CNRS, Inst Neurobiol Alfred Fessard, F-91198 Gif Sur Yvette, France - Author
Ecole Polytech, Ctr Rech Epistemol Appl, CNRS, F-75015 Paris, France - Author
Ecole Polytech, Lab Opt & Biosci, CNRS, INSERM, F-91128 Palaiseau, France - Author
Univ Politecn Madrid, Biomed Image Technol Lab ETSI Telecomunicac, E-28040 Madrid, Spain - Author
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Abstract

Investigating cell dynamics during early zebrafish embryogenesis requires specific image acquisition and analysis strategies. Multiharmonic microscopy, i.e., second-and third-harmonic generations, allows imaging cell divisions and cell membranes in unstained zebrafish embryos from 1- to 1000-cell stage. This paper presents the design and implementation of a dedicated image processing pipeline (tracking and segmentation) for the reconstruction of cell dynamics during these developmental stages. This methodology allows the reconstruction of the cell lineage tree including division timings, spatial coordinates, and cell shape until the 1000-cell stage with minute temporal accuracy and micrometer spatial resolution. Data analysis of the digital embryos provides an extensive quantitative description of early zebrafish embryogenesis.
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Keywords

3rd-harmonic generation microscopyBiologyCell lineage treeCellsEmbryoEmbryogenesisEmbryosImage reconstructionImagesIn vivo 4-d imagingMicroscopyMultiphoton microscopyPipelinesSegmentationThree dimensional displaysTimingViscous watershedZebrafish

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal IEEE TRANSACTIONS ON IMAGE PROCESSING due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2012, it was in position 13/115, thus managing to position itself as a Q1 (Primer Cuartil), in the category Computer Science, Artificial Intelligence.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2026-04-08:

  • Google Scholar: 20
  • WoS: 15
  • Scopus: 17
  • Europe PMC: 8
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-08:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 42.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 42 (PlumX).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://oa.upm.es/15972/

As a result of the publication of the work in the institutional repository, statistical usage data has been obtained that reflects its impact. In terms of dissemination, we can state that, as of

  • Views: 502
  • Downloads: 734
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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: France.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Luengo Oroz, Miguel Angel) and Last Author (SANTOS LLEO, ANDRES DE).

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Awards linked to the item

This work was supported in part by Spain's Ministry of Science and Innovation under Project TEC2010-21619-C04-03, Project TEC2008-06715-C02-02, Project CDTI-CENIT (AMIT) and Project INNPACTO (PRECISION), by Comunidad de Madrid (ARTEMIS), by French Program ANR and Program ARC, by the European Regional Development Funds (FEDER), and by Europe FP6 NEST. The associate editor coordinating the review of this manuscript and approving it for publication was Prof. David S. Taubman.
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