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June 9, 2019
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Sortin2 enhances endocytic trafficking towards the vacuole in Saccharomyces cerevisiae

Publicated to: Biological Research. 48 (UNSP 39): 39- - 2015-12-23 48(UNSP 39), DOI: 10.1186/s40659-015-0032-9

Authors:

Vásquez-Soto, B; Manríquez, N; Cruz-Amaya, M; Zouhar, J; Raikhel, NV; Norambuena, L
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Affiliations

Univ Calif Riverside, Ctr Plant Cell Biol, Dept Bot & Plant Sci - Author
Univ Chile, Ctr Plant Mol Biol, Dept Biol, Fac Sci - Author
Univ Politecn Madrid, Ctr Biotecnol & Genom Plantas - Author
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Abstract

Background: A highly regulated trafficking of cargo vesicles in eukaryotes performs protein delivery to a variety of cellular compartments of endomembrane system. The two main routes, the secretory and the endocytic pathways have pivotal functions in uni- and multi-cellular organisms. Protein delivery and targeting includes cargo recognition, vesicle formation and fusion. Developing new tools to modulate protein trafficking allows better understanding the endomembrane system mechanisms and their regulation. The compound Sortin2 has been described as a protein trafficking modulator affecting targeting of the vacuolar protein carboxypeptidase Y (CPY), triggering its secretion in Saccharomyces cerevisiae. Results: A reverse chemical-genetics approach was used to identify key proteins for Sortin2 bioactivity. A genome-wide Sortin2 resistance screen revealed six yeast deletion mutants that do not secrete CPY when grown at Sortin2 condition where the parental strain does: met18, sla1, clc1, dfg10, dpl1 and yjl175w. Integrating mutant phenotype and gene ontology annotation of the corresponding genes and their interactome pointed towards a high representation of genes involved in the endocytic process. In wild type yeast endocytosis towards the vacuole was faster in presence of Sortin2, which further validates the data of the genome-wide screen. This effect of Sortin2 depends on structural features of the molecule, suggesting compound specificity. Sortin2 did not affect endocytic trafficking in Sortin2-resistant mutants, strongly suggesting that the Sortin2 effects on the secretory and endocytic pathways are linked. Conclusions: Overall, the results reveal that Sortin2 enhances the endocytic transport pathway in Saccharomyces cerevisiae. This cellular effect is most likely at the level where secretory and endocytic pathways are merged. Them Sortin2 specificity over the endomembrane system places it as a powerful biological modulator for cell biology.
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Keywords

AlkanesulfonatesBiological transportBudding yeastChemical geneticsClathrinCortical actin cytoskeletonDissectionDynamicsEndocytosisPhenotypePlant proteinsProteinProtein transportRhodanineSaccharomyces cerevisiaeSecretory pathwaySla1pSmall moleculesSortin2TargetTrafficking modulatorVacuoles

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Biological Research due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2015, it was in position , thus managing to position itself as a Q2 (Segundo Cuartil), in the category Agricultural and Biological Sciences (Miscellaneous). Notably, the journal is positioned en el Cuartil Q3 for the agency WoS (JCR) in the category Biology.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2026-01-14:

  • WoS: 6
  • Scopus: 5
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-01-14:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 30.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 30 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 2.
  • The number of mentions on the social network X (formerly Twitter): 4 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://oa.upm.es/41453/

As a result of the publication of the work in the institutional repository, statistical usage data has been obtained that reflects its impact. In terms of dissemination, we can state that, as of

  • Views: 344
  • Downloads: 209
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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Chile; United States of America.

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Awards linked to the item

FONDECYT 11080240 and 1120289 (to BV-S, NM, MC-A and LN), PCB-MN P006-065-F (to NM and LN) and NSF MCB0515963 (to JZ and NVR).
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