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Sousaraei, NaeimehAuthor

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February 28, 2022
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Marker assisted backcrossing for introgression of Fusarium wilt resistance gene into melon

Publicated to:Euphytica. 214 (1): - 2018-01-01 214(1), DOI: 10.1007/s10681-017-2080-4

Authors: Sousaraei, Naeimeh; Ramshini, Hossein; Lotfi, Mahmoud; Sharzei, Abbas;

Affiliations

Univ Tehran, Dept Hort, Aburaihan Campus, Tehran, Iran - Author
Univ Tehran, Deptartment Agron & Plant Breeding, Aburaihan Campus, Tehran, Iran - Author
Univ Tehran, Deptartment Entomol & Plant Dis, Aburaihan Campus, Tehran, Iran - Author
Univ Tehran, Tehran, Iran - Author

Abstract

Fusarium wilt, caused by Fusarium oxysporum f. sp. melonis is a common vascular wilt fungal disease in melon across the world. The resistance gene to race 1 of this causal agent, Fom-2, has been previously cloned and its sequence is available. The objective of this research was the introgression of Fom-2 from one resistant (Isabelle) genotype into two susceptible cultivars (Garmak and Tile-torogh) via marker assisted backcrossing. First, the leucine-rich repeats (LRR) domain of Fom-2 from resistant and susceptible genotypes was sequenced to develop functional markers. A length of 1274 bp of the 30 end of this gene was isolated, cloned and sequenced. The difference between resistant and susceptible genotypes in this region was 28 nucleotide substitutions. Two allele specific primer pairs, Fom2-R409 and Fom2-S253, were designed based on nucleotide substitutions to amplify resistant and susceptible alleles, respectively. For introgression of the gene, donor (Isabelle) and recurrent (Garmak and Tile-torogh) parents were crossed. Resistant plants in BC1F1 and BC2F1 generations were first detected using artificial pathogen inoculation and later the plants were genotyped by functional markers to validate their resistance. The resistant plants were also selected phenotypically in each generation for background genome recovery, which conduced to high similarity of BC3 generation with the recurrent parents. It was proved the developed markers are more precise and efficient than inoculation trial and could be used as confident tools for screening of resistant melon genotypes to Fusarium wilt.

Keywords

Allele specific pcrCucumis meloFom-2Leucine-rich repeatsMarker assisted selectionRacesSequence characterized amplified regions

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Euphytica due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2018, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Horticulture.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-07-15:

  • WoS: 1
  • Scopus: 2

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-07-15:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 34 (PlumX).

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (SOUSARAEI, NAEIMEH) .