Marker assisted backcrossing for introgression of Fusarium wilt resistance gene into melon
Publicado en:Euphytica. 214 (1): - 2018-01-01 214(1), DOI: 10.1007/s10681-017-2080-4
Autores: Sousaraei, Naeimeh; Ramshini, Hossein; Lotfi, Mahmoud; Sharzei, Abbas;
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Resumen
Fusarium wilt, caused by Fusarium oxysporum f. sp. melonis is a common vascular wilt fungal disease in melon across the world. The resistance gene to race 1 of this causal agent, Fom-2, has been previously cloned and its sequence is available. The objective of this research was the introgression of Fom-2 from one resistant (Isabelle) genotype into two susceptible cultivars (Garmak and Tile-torogh) via marker assisted backcrossing. First, the leucine-rich repeats (LRR) domain of Fom-2 from resistant and susceptible genotypes was sequenced to develop functional markers. A length of 1274 bp of the 30 end of this gene was isolated, cloned and sequenced. The difference between resistant and susceptible genotypes in this region was 28 nucleotide substitutions. Two allele specific primer pairs, Fom2-R409 and Fom2-S253, were designed based on nucleotide substitutions to amplify resistant and susceptible alleles, respectively. For introgression of the gene, donor (Isabelle) and recurrent (Garmak and Tile-torogh) parents were crossed. Resistant plants in BC1F1 and BC2F1 generations were first detected using artificial pathogen inoculation and later the plants were genotyped by functional markers to validate their resistance. The resistant plants were also selected phenotypically in each generation for background genome recovery, which conduced to high similarity of BC3 generation with the recurrent parents. It was proved the developed markers are more precise and efficient than inoculation trial and could be used as confident tools for screening of resistant melon genotypes to Fusarium wilt.
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Impacto bibliométrico. Análisis de la aportación y canal de difusión
El trabajo ha sido publicado en la revista Euphytica debido a la progresión y el buen impacto que ha alcanzado en los últimos años, según la agencia Scopus (SJR), se ha convertido en una referencia en su campo. En el año de publicación del trabajo, 2018, se encontraba en la posición , consiguiendo con ello situarse como revista Q1 (Primer Cuartil), en la categoría Horticulture.
2025-06-01:
- WoS: 1
- Scopus: 9
- OpenCitations: 5
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Análisis de liderazgo de los autores institucionales
Existe un liderazgo significativo ya que algunos de los autores pertenecientes a la institución aparecen como primer o último firmante, se puede apreciar en el detalle: Primer Autor (SOUSARAEI, NAEIMEH) .