{rfName}
Im

Indexed in

License and use

Citations

6

Altmetrics

Grant support

This research Project has been funded by the Comunidad de Madrid through the call Research Grants for Young Investigators from Universidad Politecnica de Madrid (project MEJOR-TRIGO, APOYO-JOVENES-21-Q0U62P-86-BF6LSV) . Naima Bouabdellah is financed by an excellence grant from the Government of Algeria.

Analysis of institutional authors

Naima, BouabdellahAuthorBouabdellah, NaimaAuthorChacon, EfrainAuthorBenavente, ElenaAuthorRuiz, MagdalenaAuthorGiraldo, PatriciaAuthorPascual, LauraCorresponding Author
Share
Publications
>
Article

Image-assisted quantification of high and low molecular weight glutenin fractions in wheat by SDS-PAGE

Publicated to:Journal Of Cereal Science. 118 103977- - 2024-07-11 118(), DOI: 10.1016/j.jcs.2024.103977

Authors: Bouabdellah, Naima; Chacon, Efrain; Benavente, Elena; Ruiz, Magdalena; Giraldo, Patricia; Pascual, Laura

Affiliations

CSIC, Inst Nacl Invest & Tecnol Agr & Alimentaria INIA, Alcala de Henares 28805, Madrid, Spain - Author
Univ Politecn Madrid, Dept Biotechnol Plant Biol, Sch Agr Food & Biosyst Engn, Madrid 28040, Spain - Author

Abstract

Gluten quality in bread wheat is mainly determined by gluten strength and protein content. Traditionally gluten quality has been predicted based only on allelic variation for HMW-GS characterized by SDS-PAGE- The ratio of high to low molecular weight glutenin subunits has received less attention despite its influence on gluten properties is acknowledge. That is mainly because accurate quantification of glutenin fractions is made by RPHPLC, a more complex, expensive and time-consuming technology. In the present study, a methodology to estimate HMW/LMW glutenin subunit ratio by SDS-PAGE results was assessed. We used a segregating population derived from a cross between two Spanish bread wheat landraces (Richela Blanca and Jeja Pardilla) showing striking differences according to SDSS tests. Quantification of prolamins by RP-HPLC confirmed a wide range of variation in the HMW/LMW glutenin subunit ratio within the population. Then, we selected 22 lines that represented the available variation. Subsequently, we obtained high-quality SDS-PAGE images following a standard protocol to characterize glutenin allelic variants. Estimation of HMW/LMW-GS ratio was obtained by analyzing the intensity of HMW-GS and LMW-GS gel bands. Correlation between RP-HPLC and SDS-PAGE values was greater than 0.85 proving the suitability of the much simpler and cheaper electrophoretic-based alternative.

Keywords
Allelic variationFlouGliadinGluten prolaminsHmw to lmw glutenins ratiHmw to lmw glutenins ratioPerformanceProtein quantificationStorage proteinsWheat quality

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Journal Of Cereal Science due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Food Science.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-05-17:

  • WoS: 3
  • Scopus: 3
Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-05-17:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 10 (PlumX).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • Assignment of a Handle/URN as an identifier within the deposit in the Institutional Repository: https://oa.upm.es/84598/

As a result of the publication of the work in the institutional repository, statistical usage data has been obtained that reflects its impact. In terms of dissemination, we can state that, as of

  • Views: 50
  • Downloads: 12
Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (BOUABDELLAH, NAIMA) and Last Author (PASCUAL BAÑULS, LAURA).

the author responsible for correspondence tasks has been PASCUAL BAÑULS, LAURA.